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Iranian Journal of Basic Medical Sciences، جلد ۲۵، شماره ۹، صفحات ۱۱۳۲-۱۱۴۰
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عنوان انگلیسی Dioscin ameliorates slow transit constipation in mice by up-regulation of the BMP2 secreted by muscularis macrophages
چکیده انگلیسی مقاله Objective(s): The loss of enteric neurons has been shown to be a major cause of slow transit constipation (STC). Gut microbiota and muscularis macrophages (MMs) are associated with the enteric nervous system (ENS) development and gastrointestinal (GI) motility. This study aimed to investigate whether Dioscin (DIO) increased GI motility and inhibited neuron loss by modulating gut microbiota profile, improving inflammation in the ENS microenvironment.Materials and Methods: The STC model was established by loperamide. The alteration of the gut microbiota was analyzed by 16S rDNA sequencing. The longitudinal muscle and myenteric plexus (LMMP) from the colon were prepared for flow cytometry, immunofluorescence, western blot, and qRT-PCR. Results: DIO increased the stool number, stool water content and shortened whole gut transit time, helped to recover the gut microbial diversity and microbiota community structure, and increased the abundance of Muribaculaceae in STC mice. Compared with the STC group, the number of MMs and the level of the iNOS, IL-6, and TNFα genes were significantly decreased following DIO treatment. Moreover, DIO may increase the number of HuC/D+ neurons per ganglion by up-regulating the BMP2 secreted by MMs and activating the BMP2/p-Smad1/5/9 signaling pathway. Furthermore, the level of excitatory neurotransmitter AchE in colon tissues exhibited a substantial increase in the DIO group. However, the level of inhibitory neurotransmitter VIP was markedly decreased. Conclusion: Our results provide that DIO increases GI motility and inhibits neuron loss by modulating gut microbiota profile, improving inflammation in the ENS microenvironment and up-regulating the BMP2 secreted by MMs.
کلیدواژه‌های انگلیسی مقاله BMP2, Constipation, Dioscin, Gut microbiota, Macrophages
نویسندگان مقاله | Bingbing Ren
Department of Pediatric Surgery, General Hospital, Tianjin Medical University, Tianjin, China


| Siqi Fu
Department of Pediatric Surgery, General Hospital, Tianjin Medical University, Tianjin, China


| Yong Liu
Department of Pediatric Surgery, General Hospital, Tianjin Medical University, Tianjin, China


| JianYu Kang
Department of Pediatric Surgery, General Hospital, Tianjin Medical University, Tianjin, China


| Bo Wang
Department of Pediatric Surgery, General Hospital, Tianjin Medical University, Tianjin, China


| ZhiWei Yao
Department of Pediatric Surgery, General Hospital, Tianjin Medical University, Tianjin, China


| Hao Wang
Department of General Surgery, General Hospital, Tianjin Medical University, Tianjin, China


| DaQing Sun
Department of Pediatric Surgery, General Hospital, Tianjin Medical University, Tianjin, China
نشانی اینترنتی https://ijbms.mums.ac.ir/article_20844.html
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نوع مقاله منتشر شده Original Article
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