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Iranian Journal of Basic Medical Sciences، جلد ۲۵، شماره ۶، صفحات ۷۱۵-۷۲۲
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عنوان انگلیسی Analysis of transcriptome characteristics of UTI therapy for cerebral injury after CA/ROSC based on RNA-seq technique
چکیده انگلیسی مقاله Objective(s): To study the effects and mechanisms of ulinastatin (UTI) on brain injury caused by cardiac arrest/return of spontaneous circulation (CA/ROSC). Materials and Methods: In this study, modeling of CA/ROSC was set up in 56 Sprague Dawley (SD) rats, which were randomly divided into the model group, UTI (100000U/kg) treatment group, and control group. Each group then was divided into two subgroups: 24 hr and 72 hr. The survival rates between different groups was observed during two weeks. AimPlex multiplex immunoassays technology was performed to detect the expression of inflammatory cytokines in serum, such as IL-6 and TNF-α. RNA-sequencing (RNA-seq) transcriptome, Gene Ontology (GO), and Kyoto. Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were used to investigate the possible mechanism of UTI. Western blot and immunohistochemistry were performed to detect the expression of C-C motif chemokine ligand 2 (CCl2) and plasminogen (plg) protein expression.Results: The survival rate of the UTI group was significantly higher than the model group during two weeks. And UTI can significantly reduce the content of IL-6 and TNF-α in serum. GO and KEGG pathway enrichment analysis revealed that differentially expressed genes mainly belonged to the IL-17 signaling pathway and neuroactive ligand-receptor interaction signaling pathway. Besides, UTI can down-regulate the expression of the CCl2 inflammatory gene and up-regulate the expression of plg in the brain tissue of CA/ROSC rats. Conclusion: UTI has neuroprotective effects on brain injury after CA/ROSC. And the key mechanisms belong to the regulation of immune-inflammatory response as well as the signaling molecules and interaction. 
کلیدواژه‌های انگلیسی مقاله Brain Injuries, Cardiopulmonary - Resuscitation, Heart Arrest, RNA-Seq, Urinastatin
نویسندگان مقاله | Xiaojie Bai
Department of Emergency, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, PR China


| Mingya Yang
Department of Hematology, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, PR China


| Tiantian Zhu
Department of Emergency, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, PR China


| Jun Xu
Department of Emergency, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, PR China


| Wei Wang
Department of General Surgery, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, PR China


| Yuanyuan He
Department of Emergency, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, PR China


| Yu Liu
Department of General Surgery, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, PR China


| Xingxing Li
Department of Emergency, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, PR China


| Miao He
Department of Emergency, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, PR China


| Tao Meng
Department of General Surgery, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, PR China


| Zhenzhen Wang
Department of General Surgery, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, PR China


| Hong Zhang
Department of Emergency, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, PR China


| Lixin Zhu
Department of General Surgery, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, PR China
نشانی اینترنتی https://ijbms.mums.ac.ir/article_20410.html
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