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Iranian Journal of Basic Medical Sciences، جلد ۲۵، شماره ۴، صفحات ۴۳۵-۴۴۱
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عنوان انگلیسی Neutrophil depletion reduces interstitial cajal-like cell injury and alleviates inflammation-induced motor dysfunction in guinea-pig gallbladder during acute cholecystitis
چکیده انگلیسی مقاله Objective(s): Gallbladder interstitial Cajal-like cells (ICLCs) are known as some of the players in the complex motility mechanisms affecting gallbladder motility. This study aims to explore the mechanism of guinea-pig gallbladder motility disorders during Acute Cholecystitis (AC), focusing on the relationships between neutrophil alterations, gallbladder ICLCs, and smooth muscle contractility.Materials and Methods: Forty-eight guinea pigs were randomly divided into four groups: normal, sham, common bile duct ligation (CBDL), and anti-PMN (anti-polymorphonuclear antibody treated +CBDL). Hematoxylin and eosin-stained slides from each gallbladder sample were examined for inflammation, and myeloperoxidase (MPO) activity was evaluated. The contractile response of gallbladder muscle to Ach, CCK-8, and KCl was registered by a tension transducer, and ultrastructure features of ICLCs were observed.Results: Pretreatment with anti-PMN significantly reduced the circulating neutrophils by 80% and also considerably decreased the gallbladder MPO activity by 52.9% compared with the CBDL group (P< 0.05). After adding Ach, CCK-8, and KCl, the contraction ability in CBDL and anti-PMN groups was lower than those of normal and sham groups (P< 0.05), and they were increased substantially in the anti-PMN group compared with the CBDL group (P< 0.05). Transmission electron microscopy confirmed that the cytoplasm of the neutrophils was full of granules, and neutrophils contacted closely with ICLCs. The ultrastructure of ICLCs in the anti-PMN group was less inflamed and the endoplasmic reticulum was mildly dilated, and cell processes also increased.Conclusion: Anti-PMN could relieve the ultrastructure injury of ICLCs and alleviate gallbladder dysmotility during AC. Neutrophils may damage gallbladder ICLCs at first followed by dysmotility.
کلیدواژه‌های انگلیسی مقاله Common bile duct ligation, Gallbladder dysmotility, Guinea pigs, Interstitial cajal-like cells, Neutrophils, Ultrastructure features
نویسندگان مقاله | Li Zhang
Department of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, No. 1, Youyi Road, Yuzhong District, Chongqing 400016, China


| Bin Yang
Department of Gastroenterology, Renmin Hospital of Wuhan University, No. 238, Jiefang Road, Wuhan 430060, Hubei Province, China


| Yong Xiao
Department of Gastroenterology, Renmin Hospital of Wuhan University, No. 238, Jiefang Road, Wuhan 430060, Hubei Province, China


| Bingqiang Zhang
Department of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, No. 1, Youyi Road, Yuzhong District, Chongqing 400016, China


| Baoping Yu
Department of Gastroenterology, Renmin Hospital of Wuhan University, No. 238, Jiefang Road, Wuhan 430060, Hubei Province, China


| Youlin Kuang
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, No. 1, Youyi Road, Yuzhong District, Chongqing 400016, China
نشانی اینترنتی https://ijbms.mums.ac.ir/article_20008.html
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