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Iranian Journal of Medical Sciences، جلد ۴۷، شماره ۶، صفحات ۵۵۸-۵۶۵
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عنوان انگلیسی Production of a Novel Multi-Epitope Peptide Vaccine against Hepatocellular Carcinoma
چکیده انگلیسی مقاله Background: Hepatocellular carcinoma (HCC) is one of the prevalent cancers in the world with a high recurrence rate. In recent years, different researches have focused on designing efficient multi-epitope peptide vaccines against HCC. In designing these vaccines, over-expressed antigens in HCC patients, such as α- fetoprotein (AFP) and glypican-3 (GPC-3), have been employed. In our previous study, a multi-epitope peptide vaccine for HCC was designed by in-silico methods. The designed vaccine construct included the AFP, GPC-3, and aspartyl-β-hydroxylase (ASPH) as CytoLoxic T cell Lymphocytes (CTL), one epitope from Tetanus Toxin Fragment C (TTFrC) as Helper T cell Lymphocytes (HTL), and a segment of microbial heat shock protein (HSP70) peptide407-426 as an adjuvant. All the mentioned parts were connected by appropriate linkers. The aim of this study is the production of the designed vaccine. Methods: This research is experimental and was carried out in Fasa, Iran, in 2017. The designed vaccine construct gene was transformed to the Escherchia coli BL21 (DE3) strain and expressed in different isopropyl β-D-1-thiogalactopyranoside (IPTG) concentrations (0.6 and 1 mM), times (4, 6, 8, 16 hours), and temperatures (25 and 37 °C). Then, the expressed protein was analyzed by Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and the Western blot methods. Results: The best conditions for protein expression were obtained in the Super Optimal Broth (SOB) medium at 37 °C after the induction of expression by 1 mM IPTG for six hour. Conclusion: The recombinant HCC vaccine was produced with a proper concentration. 
کلیدواژه‌های انگلیسی مقاله Hepatocelluar carcinoma, Vaccine, Subunit, expression, Designed
نویسندگان مقاله Fatemeh Motamedi Dehbarez |
Department of Medical Biotechnology, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran

Shirin Mahmoodi |
Department of Medical Biotechnology, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran

نشانی اینترنتی https://ijms.sums.ac.ir/article_48283_edb6ea0517807c0dc2fab8c8fc92949c.pdf
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