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Caspian Journal of Enviromental Sciences، جلد ۲۰، شماره ۴، صفحات ۸۱۳-۸۱۹
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عنوان انگلیسی Detecting genetics of several isolated bacterial species from soils by hydrocarbons
چکیده انگلیسی مقاله The presence of hydrocarbons in the soil is considered one of the main problems of pollution. In our current study, eight samples isolated from soil saturated with hydrocarbons were taken from different areas of Baghdad, Iraq. In this study, 5 isolates belonging to Pseudomonas aeruginosa by 99%, 4 isolates to Klebsiella pneumoniae by 98%, and 3 isolates to Enterobacter hormaechei by 97% were diagnosed in different ways. A molecular examination was also conducted by 16sRNA. We recorded P. aeruginosa, K. Pneumoniae and E. hormaechei as new local isolates in NCBI. In addition, a comparison was made between our isolates and the global isolates to determine the degree of convergence in the evolutionary line. The genes alkB and nahAc7 were diagnosed in P. aeruginosa capable of degradation hydrocarbons. The aim of this study was to identify the bacterial species that resist the presence of hydrocarbons in the soil and also to diagnose some genes in the bacteria responsible for degradation of hydrocarbons in order to find the biological treatment methods.
کلیدواژه‌های انگلیسی مقاله hydrocarbon, Pseudomonas aeruginosa, 16sRNA, alkB gene, nahAc7 gene
نویسندگان مقاله Rana AH AL-Lami |
Biology Department, College of Science for Women, University of Baghdad, Baghdad, Iraq

Ithar Kamil Al-Mayaly |
Biology Department, College of Science, University of Baghdad, Baghdad, Iraq

نشانی اینترنتی https://cjes.guilan.ac.ir/article_5771_25431ac12c37eb171cdfcbbcccb83bd9.pdf
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