| چکیده انگلیسی مقاله |
Objective(s): To explore the effects and mechanism of three types of fresh Rehmannia glutinosa, namely Beijing No. 3 (BJ3H), Huaizhong No. 1 (HZ1H), and Taisheng (TS) on lipopolysaccharide (LPS)-induced acute kidney injury in the sepsis (S-AKI) mice model through the estrogen receptor pathway.Materials and Methods: BALB/c mice were randomly divided into control (CON), model (LPS), astragalus injection (ASI), BJ3H, HZ1H, TS water extract groups, the estrogen receptor antagonist ICI182,780 groups were added to each group. The antagonist groups received an intraperitoneal injection of ICI 0.5 hr before administration and an intraperitoneal injection of LPS 3 days after administration. The kidney pathology, function, inflammatory factors, immune cells, levels of reactive oxygen species (ROS), apoptosis, and the protein expression levels of TLR4/NF-κB/NLRP3 signaling pathway in the mice kidneys were detected.Results: ASI, BJ3H, HZ1H, and TS improved LPS-induced renal pathology in S-AKI mice, reduced the kidney and serum levels of inflammatory factors, positive rates of macrophages and neutrophils, levels of ROS and apoptosis, and the relative expression levels of TLR4, MyD88, NF-κB p-p65/NF-κB p65, and NLRP3 proteins in the kidney. In addition, they increased the positive rate of dendritic cells (DCs) in the mice kidneys. The overall effect of HZ1H was superior to that of ASI, BJ3H, and TS. However, after adding ICI, the regulatory effects of drugs were inhibited.Conclusion: The three types of fresh R. glutinosa may completely or partially affect the TLR4/NF-κB/NLRP3 signaling pathway through the estrogen receptor pathway to exert a protective effect on S-AKI. |
| نویسندگان مقاله |
| Meng Liu
Department of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China|The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China
| Mengnan Zeng
Department of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China|The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China
| Pengli Guo
Department of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China|The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China
| Yuhan Zhang
Department of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China|The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China
| Xiaofeng Yang
Department of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China|The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China
| Jufang Jia
Department of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China|The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China
| Qinqin Zhang
Department of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China|The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China
| Beibei Zhang
Department of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China|The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China
| Bing Cao
Department of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China|The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China
| Ru Wang
Department of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China|The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China
| Xiaoke Zheng
Department of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China|The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China
| Weisheng Feng
Department of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China|The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China
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