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Research in Pharmaceutical Sciences، جلد ۱۸، شماره ۳، صفحات ۳۱۷-۳۲۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Bioassay-guided isolation in Salvia abrotanoides Karel. stem based on its anti-fungal and anti-trichomonas activity
چکیده انگلیسی مقاله Background and purpose Salvia abrotanoides is considered a medicinal plant and has a broad distribution in Iran. In Iran's traditional medicine, it is also used to treat leishmaniasis, malaria, atherosclerosis, cardiovascular disease, and as a disinfectant. This research aimed to determine the anti- Candida component from S. abratonoides and anti- Trichomonas natural compounds from the stems of this plant. Experimental approach: The plant shoots were collected, dried, and after removing the leaves, grounded. Dried plant material was extracted in a maceration tank, concentrated by a Rotavap, degreased, and fractionated by normal column chromatography. Based on anti-fungal screening against Candida species, Fr. 4, with more anti-fungal activity, was selected for phytochemical analysis, by different chromatographic methods on the silica gel column and Sephadex LH-20. Isolated compounds were elucidated by NMR analysis, mass spectrum, and ultraviolet spectroscopy. Anti-fungal effects were investigated using the fungal suspension, incubation, and parasite-counting methods on purified compounds. Antibacterial effects were assessed using the Broth dilution test and reported according to the MIC parameter. Findings/Results: Two diterpenoid compounds named carnosol (compound 1), 11-hydroxy-12-methoxy-20-norabiata-8, 11, 13-trien (compound 2), and a flavonoid: 6,7-dimethoxy-5, 4'-dihydroxyflavone (compound 3) were isolated and identified. Compound 1 had selective anti-fungal effects against C. albicans , C. glabrata, and C. parapsilosis , but weak toxicity against Trichomonas vaginalis with IC 50 of 675.8 μg/mL, less than metronidazole with an IC 50 of 13.2 μg/mL. Conclusion and implications: Carnosol as the main component was assayed against Candida , Aspergillus , Rhizopus , and Trichomanas species. The results confirmed its effect on Candida compared to standard drugs.
کلیدواژه‌های انگلیسی مقاله Candida,Carnosol,Salvia,Trichomonas.
نویسندگان مقاله | Mustafa Ghanadian
Department of Pharmacognosy, Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Rasoul Jahanshahi Afshar
Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Hamed Fakhim
Department of Parasitology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Hosseinail Yousefi
Department of Pharmaceutical Biology and Botany, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland.


| Adam Matkowski
Core Research Facilities, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Mohammad Khodadadi
Core Research Facilities, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Shima Gharibi


نشانی اینترنتی http://rps.mui.ac.ir/index.php/jrps/article/view/2192
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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