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Molecular Biology Research Communications، جلد ۱۲، شماره ۱، صفحات ۱۷-۲۵
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عنوان انگلیسی The molecular characteristics of colorectal cancer: Impact of Ibuprofen and hyperthermia
چکیده انگلیسی مقاله Despite various treatment options available for colorectal cancer, the survival rates for patients remain low. This study investigated the effects of hyperthermia and Ibuprofen on human colorectal adenocarcinoma cells (HT-29) viability, proliferation, and gene expression related to tumor suppression, Wnt signaling pathways, proliferation, and apoptosis The cells were exposed to hyperthermia at 42 or 43°C for 3 hours or Ibuprofen at different concentrations (700-1500 μM), and the effects were analyzed through MTT assay, trypan blue staining, and quantitative Real-time PCR. The study used quantitative Real-time PCR (qRT-PCR) to evaluate the effect of hyperthermia and Ibuprofen on the expression of various genes associated with tumor suppression, proliferation, Wnt signaling pathway, and apoptosis. The results revealed that hyperthermia caused a minor reduction in the viability and proliferation of HT-29 cells, but the decrease was not statistically significant (P< 0.05). On the other hand, Ibuprofen caused a concentration-dependent decrease in the viability and proliferation of HT-29 cells. Both hyperthermia and Ibuprofen reduced the expression of WNT1, CTNNB1, BCL2, and PCNA genes, and increased the expression of KLF4, P53, and BAX genes. However, the changes in gene expression were not statistically significant in cells treated with hyperthermia. The findings suggest that Ibuprofen is more effective in reducing cancer cell proliferation by promoting apoptosis and inhibiting the Wnt signaling pathway than hyperthermia, which had some impact but was not statistically significant. The study highlights the potential of Ibuprofen as a targeted therapy for colorectal cancer.
کلیدواژه‌های انگلیسی مقاله Colorectal cancer, Hyperthermia, HT29 Cells, Ibuprofen, Wnt Signaling Pathway
نویسندگان مقاله Farzaneh Zarghampoor |
Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

Behnaz Valibeigi |
Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran

Abbas Behzad-Behbahani |
Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

نشانی اینترنتی https://mbrc.shirazu.ac.ir/article_6973_cc151bdcff12805a83af02b55ff63552.pdf
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