| چکیده انگلیسی مقاله |
Background and purpose: Topical antibiotic medication is an alternative method in treatment of local infections, especially osteomyelitis. Currently several biomaterials are used for this purpose. The present study focused on the fabrication and characterization of chitosan and Vancomycin (VCM)-loaded chitosan (CS)-hydroxyapatite (HA)-gelatin (G) bead in treatment of osteomyelitis. Materials and methods: CS/G/HA/VCM scaffold was prepared by cross-linking using ionotropic gelling. The morphological and mechanical properties, loading efficiency and antibacterial properties were investigated and toxicity assay of fabricated structure was also analyzed. Results: The lyophilization reduced the area, environment, and sphericity of beads. CS/G/HA/VCM structure showed smaller area (4.5 mm2) and perimeter (180 µm) compared to other structures. Suitable morphology and porosity in fabricated beads were confirmed by SEM images in CS/G/HA and CS/G structures. Reduction of strength was observed after adding VCM into fabricated structure, but this happened less in CS/G/HA/VCM structure. Release on day 14 was 58% for CS/G/HA/VCM structure. AddingVCM into CS/VCM (1.88 Mpa) and CS/G/VCM (10.46 Mpa) structures decreased the mechanical strength while it increased the mechanical strength in CS/G/HA/VCM (83.21 Mpa) structure. No toxic activity of fabricated structures was seen against NIH 3T3 cells. The results of the antimicrobial activity against Staphylococcus aureus showed that chitosan beads containing VCM (CS/VCM, CS/G/VCM, CS/G/HA/VCM) could significantly inhibit the growth of S. aureus compared to the positive control (VCM 5%)(P< 0.001). onclusion: According to current study, the CS/G/HA/VCM structure can be introduced as a promising candidate in treatment of osteomyelitis. |
| نویسندگان مقاله |
نفیسه جیرفتی | Nafiseh Jirofti
PhD in Chemical Engineering, Orthopedic Research Center, Department of Orthopedic Surgery, Mashhad University of Medical Science, Mashhad, Iran. Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
دکترای تخصصی مهندسی شیمی، گروه ارتوپدی، دانشکده پزشکی، مرکز تحقیقات ارتوپدی، دانشگاه علوم پزشکی مشهد، مشهد، ایران . مرکز تحقیقات بیوتکنولوژی، پژوهشکده فناوری دارویی، دانشگاه علوم پزشکی مشهد، مشهد، ایران
آزاده شاهرودی | Azadeh Shahroodi
MSc in Microbiology, Department of Pharmaceutics, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
کارشناسی ارشد میکروب شناسی، گروه فارماسیوتیکس، دانشکده داروسازی، دانشگاه علوم پزشکی مشهد، مشهد، ایران
جبرائیل موفق | Jebreil Movaffagh
Professor, Department of Pharmaceutics, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran . Professor, Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
استاد، گروه فارماسیوتیکس، دانشکده داروسازی، دانشگاه علوم پزشکی مشهد، مشهد، ایران
بی بی صدیقه فضلی بزاز | Bibi Sedigheh Fazly Bazzaz
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.Professor, Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
مرکز تحقیقات بیوتکنولوژی، پژوهشکده فناوری دارویی، دانشگاه علوم پزشکی مشهد، مشهد، ایران.استاد،گروه کنترل داروئی، دانشکده داروسازی، دانشگاه علوم پزشکی مشهد، مشهد، ایران
رضوان یزدیان رباطی | Rezvan Yazdian-Robati
Assistant Professor, Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran
استادیار، مرکز تحقیقات علوم دارویی، پژوهشکده هموگلوبینوپاتی، دانشگاه علوم پزشکی مازندران، ساری، ایران
مریم هاشمی | Maryam Hashemi
Assistant Professor, Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran . Nanotechnology Research Center, Pharmaceutical Technology Institute Mashhad University of Medical Sciences, Mashhad, Iran
استادیار، گروه بیوتکنولوژی دارویی، دانشکده داروسازی دانشگاه علوم پزشکی مشهد، مشهد، ایران . مرکز تحقیقات نانوفناوری، پژوهشکده فناوری دارویی، دانشگاه علوم پزشکی مشهد، مشهد، ایران
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