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Iranian Journal of Basic Medical Sciences، جلد ۱۹، شماره ۱۲، صفحات ۱۲۷۱-۱۲۷۸
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی Survival, proliferation, and migration of human meningioma stem-like cells in a nanopeptide scaffold
چکیده انگلیسی مقاله Objective(s): In order to grow cells in a three-dimensional (3D) microenvironment, self-assembling peptides, such as PuraMatrix, have emerged with potential to mimic the extracellular matrix. The aim of the present study was to investigate the influence of the self-assembling peptide on the morphology, survival, proliferation rate, migration potential, and differentiation of human meningioma stem-like cells (hMgSCs). Materials and Methods: The efficacy of a novel method for placing hMgSCs in PuraMatrix (the injection approach) was compared to the encapsulation and surface plating methods. In addition, we designed a new method for measurement of migration distance in 3D cultivation of hMgSCs in PuraMatrix. Results: Our results revealed that hMgSCs have the ability to form spheres in stem cell culture condition. These meningioma cells expressed GFAP, CD133, vimentin, and nestin. Using the injection method, a higher proliferation rate of the hMgSCs was observed after seven days of culture. Furthermore, the novel migration assay was able to measure the migration of a single cell alone in 3D environment. Conclusion: The results indicate the injection method as an efficient technique for culturing hMgSCs in PuraMatrix. Furthermore, the novel migration assay enables us to evaluate the migration of hMgSCs.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله سجاد سحاب نگاه | sajad sahab negah
histology and embryology group, basic science department, faculty of veterinary medicine, shiraz university, shiraz, iran|shefa neuroscience research center, khatam alanbia hospital, tehran, iran

سازمان اصلی تایید شده: مرکز تحقیقات علوم اعصاب شفا (Shefa neuroscience research center)

هادی علیقلی | hadi aligholi
department of neuroscience, school of advanced medical sciences and technologies, shiraz university of medical sciences, shiraz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

ذبیح اله خاکسار | zabihollah khaksar
histology and embryology group, basic science department, faculty of veterinary medicine, shiraz university, shiraz, iran

سازمان اصلی تایید شده: دانشگاه شیراز (Shiraz university)

هادی کاظمی | hadi kazemi
pediatric department, medical faculty, shahed university, tehran, iran

سازمان اصلی تایید شده: دانشگاه شاهد (Shahed university)

سید مصطفی مدرس موسوی | sayed mostafa modarres mousavi
shefa neuroscience research center, khatam alanbia hospital, tehran, iran

سازمان اصلی تایید شده: مرکز تحقیقات علوم اعصاب شفا (Shefa neuroscience research center)

مریم صفاهانی | maryam safahani
shefa neuroscience research center, khatam alanbia hospital, tehran, iran|department of community nutrition, school of nutritional sciences and dietetics, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

پرستو براتی دوم | parastoo barati dowom
shefa neuroscience research center, khatam alanbia hospital, tehran, iran

سازمان اصلی تایید شده: مرکز تحقیقات علوم اعصاب شفا (Shefa neuroscience research center)

علی گرجی | ali gorji
shefa neuroscience research center, khatam alanbia hospital, tehran, iran|epilepsy research center, westfälische wilhelms-universität münster, germany|department of neurology and department of neurosurgery, westfälische wilhelms-universität münster, germany|department of neuroscience, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_7907.html
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-274582.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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