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Iranian Journal of Basic Medical Sciences، جلد ۱۹، شماره ۱۲، صفحات ۱۲۹۲-۱۲۹۸
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عنوان انگلیسی Polysaccharide from Sepia esculenta ink and cisplatin inhibit synergistically proliferation and metastasis of triple-negative breast cancer MDA-MB-231 cells
چکیده انگلیسی مقاله Objective(s): This paper aims to investigate synergistic inhibition of polysaccharide from Sepia esculenta ink (SIP), a newly isolated marine polysaccharide in our laboratory, on breast cancer MDA-MB-231 cells exposed to cisplatin. Materials and Methods: Cell viability of MDA-MB-231 cells was determined by CCK 8 assay. Median-effect concentration was analyzed using Chou-Talalay method that was also subjected to determine cell inhibition ratio and combined index, as well as interaction between SIP and cisplatin. Proliferation and migration abilities were detected with plate colony formation assay and cell wound scratch assay, respectively. Expression of MMP-2 and MMP-9 proteins was measured with Western blot assay. Results: Data showed that SIP not only suppressed proliferation and migration of MDA-MB-231 cells, and expression of MMP-2 and MMP-9 proteins, also promoted inhibition of cisplatin on proliferation, migration and MMPs expression of MDA-MB-231 cells, which indicates synergy inhibition of drug combination of SIP and cisplatin on breast cancer cells. The median-effect concentrations of cisplatin and SIP were 4.9 and 1659.6 μg/ml, respectively. Whereas the concentration of combination drug was 158.5 μg/ml. The data indicated that drug combination can decrease dosages of the two single agents, especially the usual dosage of cisplatin. Conclusion: This research demonstrated that SIP repressed proliferation and metastasis of MDA-MB-231 cells and promoted anticancer effect of cisplatin on the breast cancer cells. The data suggested that SIP is a potential natural drug that can be used as an auxiliary medicine alongside chemotherapy in treating breast cancer.
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نویسندگان مقاله hua zhong liu | hua zhong liu
college of chemistry and environment, guangdong ocean university, zhanjiang 524088, china


wei محمد بن همام رییس کنفدراسیون | wei xiao
guangxi buffalo research institute, chinese academy of agricultural sciences, nanning 530001, china|college of food science amp;amp; technology, guangdong ocean university, zhanjiang 524088, china


yi peng gu | yi peng gu
institute of food science amp;amp; engineering technology, hezhou university, hezhou 542899, china


ye xing تایو | ye xing tao
experimental center for sciences, guilin medical university, guilin 541004, china


da یان زانگ | da yan zhang
college of food science amp;amp; technology, guangdong ocean university, zhanjiang 524088, china


هیو du | hui du
biochemisty center, guangdong ocean university, zhanjiang 524088, china


jiang hua shang | jiang hua shang
guangxi buffalo research institute, chinese academy of agricultural sciences, nanning 530001, china
نشانی اینترنتی http://ijbms.mums.ac.ir/article_7913.html
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-274585.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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