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Iranian Journal of Basic Medical Sciences، جلد ۱۹، شماره ۱۲، صفحات ۱۳۲۵-۱۳۳۰
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عنوان انگلیسی Molecular typing of nosocomial Staphylococcus aureus strains associated to biofilm based on the coagulase and protein A gene polymorphisms
چکیده انگلیسی مقاله Objective(s): Staphylococcus aureus is an important bacterial pathogen responsible for a variety numbers of nosocomial and community acquired infections. Biofilm formation is regarded as an important factor in the establishment of S. aureus infection. The contribution of the genetic background of S. aureus to biofilm formation is poorly understood. The aim of the present work was to genotype S. aureus strains associated to biofilm based on the coagulase and protein A genes and to evaluate the association between the genetic background and the biofilm forming ability of clinical S. aureus isolates. Materials and Methods: A total number of 100 S. aureus were isolated from nosocomial infections and biofilm formation capability was investigated using phenotypic assay and molecular detection of biofilm associated genes. The strains were genotyped based on coagulase (coa) and protein A (spa) gene polymorphisms using restriction fragments length polymorphism-polymerase chain reaction (RFLP-PCR). Results: RFLP-PCR of coa gene generated two types and three subtypes. Amplification of spa gene resulted in two banding patterns and their restriction digestion generated three subtypes. The combined coa and spa RFLP patterns generated nine genotypes (G1-G9). The genotypes G4 and G1 were the most prevalent (32.1% and 24.3%, respectively). Conclusion: High clonal diversity of S. aureus strains able to produce biofilm was observed. Biofilm formation correlates with the spa and coa clonal lineage in our population and testing for multiple gene polymorphisms could be employed for local epidemiologic purposes.
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نویسندگان مقاله علی صالح زاده | ali salehzadeh
department of biology, rasht branch, islamic azad university, rasht, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

حجت اله زمانی | hojjatolah zamani
department of biology, faculty of science, university of guilan, rasht, iran

سازمان اصلی تایید شده: دانشگاه گیلان (Guilan university)

مایده کشتکار لنگرودی | maedeh keshtkar langeroudi
department of biology, rasht branch, islamic azad university, rasht, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

امیر میرزایی | amir mirzaie
young researchers and elite club, east tehran branch, islamic azad university, tehran, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_7919.html
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-274589.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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