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Iranian Journal of Basic Medical Sciences، جلد ۱۹، شماره ۱۲، صفحات ۱۳۳۱-۱۳۳۶
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چکیده فارسی مقاله
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عنوان انگلیسی MicroRNA-297a regulates vascular calcification by targeting fibroblast growth factor 23
چکیده انگلیسی مقاله Objective(s): Vascular calcification is one the major characteristics in patients with various types of chronic inflammatory disorders. MiRNAs have been shown to be involved in many normal biological functions as well as diseases; however, their role in vascular calcification has not received much attention. Materials and Methods: In the current study, we built a vascular calcification rat model using vitamin D3 plus nicotine and analyzed miRNA expression profile by miRNA chip assay. Potential target of one selected miRNA with sharpest variation in expression were predicted by both PicTar and TargetScan. The impact of the selected miRNA on the expression of the potential target on both mRNA and protein levels were measured by RT-PCR and Western blot, respectively. Results: Our results identified 16 dysregulated miRNAs, among which miR-297a showed the sharpest variation. Further analysis focusing on miR-297a revealed that fibroblast growth factor 23 (FGF23) was a potential target of miR297a. Measurement of FGF23 and its regulator Klotho on both mRNA and protein levels demonstrated that FGF23 was significantly increased while Klotho was decreased in rats with vascular calcification. Conclusion: Our results indicated that FGF23 was target of miR-297a and decreased miR-297a in vascular calcification lead to the increase of FGF23, which together with Klotho might enhance vascular calcification. The findings of this study could provide valuable information for the understanding of mechanisms underlying miR-dependent vascular calcification as well as potential treatment target for the disease.
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نویسندگان مقاله shouhua zheng | shouhua zheng
department of general surgery, the first affiliated hospital of zhengzhou university, zhengzhou, henan, 450052 china


shuijun زانگ | shuijun zhang
department of general surgery, the first affiliated hospital of zhengzhou university, zhengzhou, henan, 450052 china


یان song | yan song
department of general surgery, the first affiliated hospital of zhengzhou university, zhengzhou, henan, 450052 china


wenzhi گیو | wenzhi guo
department of general surgery, the first affiliated hospital of zhengzhou university, zhengzhou, henan, 450052 china


wenlong جای | wenlong zhai
department of general surgery, the first affiliated hospital of zhengzhou university, zhengzhou, henan, 450052 china


xinguang qiu | xinguang qiu
department of general surgery, the first affiliated hospital of zhengzhou university, zhengzhou, henan, 450052 china


jianhua li | jianhua li
department of general surgery, the first affiliated hospital of zhengzhou university, zhengzhou, henan, 450052 china
نشانی اینترنتی http://ijbms.mums.ac.ir/article_7920.html
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-274590.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
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