این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Nanomedicine Journal، جلد ۱۰، شماره ۳، صفحات ۱۹۷-۲۰۹
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Effect of polycaprolactone/carbon nanotube scaffold implantation along with liposomal ellagic acid in hippocampal synaptogenesis after spinal cord injury
چکیده انگلیسی مقاله Objective(s): Memory and cognition impairments are the most important secondary effects of spinal cord injury (SCI) in the hippocampus. Therefore, the present study aimed to examine the effect of implantation of polycaprolactone/ functionalized multiwall carbon nanotube (PCL/f-MWCNT) scaffold along with ellagic acid loaded liposome (EA@lip) in neurological function recovery and hippocampus deficit after SCI.Materials and Methods: Twenty-four female Wistar rats were randomly assigned into 4 groups (n=6): Ctrl- group (laminectomy without SCI), Ctrl+ group (SCI), PCL/CNT group (implantation of PCL/f-MWCNT scaffold) and PCL/CNT/EA group (implantation of PCL/f-MWCNT/EA@lip scaffold). The injury model was the dorsal hemisection at the T9 level. Characterization of EA@lip made by remote loading method was done by transmission electron microscopy and dynamic light scattering. Also, the morphology of PCL/f-MWCNT fibers was investigated by field-emission scanning electron microscopy (FE-SEM). Behavioral tests were used to evaluate the neurobehavioral performance of the animals. After 4-weeks, excitatory postsynaptic potential was recorded from the CA1 area of the hippocampus. Hippocampal mRNA levels of amyloid beta precursor protein (APP), cyclic nucleotide phosphodiesterase (CNP), glutamate ionotropic receptor kainate type subunit 2 (GRIK2) and syntaxin-binding protein 1 (Munc 18-1) were assayed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR).Results: We demonstrated that, after implanting the PCL/CNT scaffold with or without EA@lip, the hippocampal action potential improved by increasing the slope and amplitude of fEPSP compared to the Ctrl+ group. RT-qPCR data showed that the expression of CNP and Munc 18-1 increased, and the expression of APP and GRIK2 decreased, in the groups that received PCL/CNT with or without EA@lip compared to the injury group. We also proved that the treatment with PCL/CNT/EA@lip improved behavioral performance compared to the Ctrl+ and PCL/CNT groups.Conclusion: This study demonstrated that PCL/f-MWCNT/EA@lip scaffold implantation improves functional potential and alters the expression of memory-related genes in the hippocampus post-injury.
کلیدواژه‌های انگلیسی مقاله Action potentials, Drug delivery systems, Memory, Neurogenesis, Tissue engineering 
نویسندگان مقاله | Arman Abroumand Gholami
Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran|Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran


| Fatemeh Gheybi
Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran|Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran


| Amir Mahdi Molavi
Department of Materials Research, Iranian Academic Center for Education, Culture and Research, Khorasan Razavi Branch, Mashhad, Iran


| Fatemeh Tahmasebi
Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


| Arash Papi
Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran


| Hamideh Babaloo
Regenerative Medicine, Organ Procurement and transplantation Multi-Disciplinary Center, Razi Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
نشانی اینترنتی https://nmj.mums.ac.ir/article_22560.html
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده مقاله پژوهشی
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات