این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Physiology and Pharmacology، جلد ۲۷، شماره ۲، صفحات ۱۹۲-۲۰۱
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Estradiol preserves synapse-related proteins against oligomeric amyloid beta in the hippocampal cultured cells: possible involvement of protein kinase M zeta
چکیده انگلیسی مقاله Introduction: Estradiol has been shown to facilitate synaptic long-term potentiation (LTP) mainly through translocation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor from intracellular pool to the post synaptic membrane. On the other hand, oligomeric amyloid beta (oAβ) decreases number of AMPA receptors in the synapses. It is well known that trafficking of AMPA receptors is governed by an atypical and autonomously active isoform of PKC called protein kinase M zeta (PKMζ). In spite of these evidence, the effect of estradiol on PKMζ expression is not yet studied. We aim to examine the possible protective effect of estradiol on PKMζ and AMPA receptor subunits against oAβ in hippocampal primary cell culture. Methods: Primary cell culture was prepared from postnatal (P0 to P3) rat pups. They were decapitated and the brains were removed. Hippocampi were isolated and collected in cold phosphate buffer saline. Then, they were trypsinized at 37°C for 15min. The cells were treated with 1µM OAβ or vehicle for 24h and then with 100nM estradiol for another 24h. Using the western blot analysis, the expression level of AMPAR subunit glutamate receptor 1 (GluA1), GluA2 and PKMζ were determined. Results: OAβ decreased the level of GluA1, GluA2 and PKMζ. Estradiol did not change the molecule levels in healthy cells; however, it preserved their expression levels in OAβ treated cells. Conclusion: These findings suggest that estradiol may restore expression level of synapse related molecules in an Alzheimer' disease cell model, in part, through acting on PKMζ signaling pathway.
کلیدواژه‌های انگلیسی مقاله Alzheimer’s disease,estradiol,PKMζ,amyloid beta,AMPA
نویسندگان مقاله | Sogol Atabaki-Mehr
Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, IranDepartment of Cell and Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran


| Saeed Airian
Department of Cell and Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran


| Hamid Gholami Pourbadie
Pasteur Institute of Iran
نشانی اینترنتی http://ppj.phypha.ir/browse.php?a_code=A-10-1504-1&slc_lang=en&sid=1
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Neurophysiology/Pharmacology
نوع مقاله منتشر شده Experimental research article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات