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Addiction and Health، جلد ۱۵، شماره ۲، صفحات ۸۷-۹۲
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عنوان انگلیسی Promoter Methylation of Two HOXA9 and NISCH Genes in Opium Users
چکیده انگلیسی مقاله Background: Opiate abuse has been critically increased in the world, especially in Iran. Owing to the association of opiate use with multiple human cancers and neurological disorders, seeking for genetic and epigenetic effects of opium can pave the way for early diagnosis of major health defects in addicted users. Accordingly, the present study aimed to determine the methylation status of the promoter of two genes, which are actively involved in neurodevelopment and cancer evolution.Methods: DNA was isolated from peripheral blood of 28 opium abusers and 19 healthy controls and then subjected to sonication. Sonicated DNAs undergone methylated DNA immunoprecipitation-real time polymerase chain reaction (MeDIP-Real Time PCR) using specific primer pairs designed for HOXA9 and NISCH genes. Obtained data were analyzed using SPSS software.Findings: HOXA9 and NISCH genes were found to be significantly methylated in addicted users compared to controls (P< 0.001) which was significantly associated with the mean of the age regarding HOXA9 gene (P=0.002). Neither opium amount nor duration or route of using was associated with the methylation status of HOXA9 or NISCH genes.Conclusion: Hypermethylation of HOXA9 and NISCH genes as tumor suppressor in opium-addicted individuals can be considered as confirmatory evidence for carcinogenesis of opium. Further studies are required to figure out the role of epigenetic alterations in cancer evolution among opium users.
کلیدواژه‌های انگلیسی مقاله HOXA9, NISCH, Promoter methylation, Opium, MeDIP-Real Time PCR
نویسندگان مقاله Majid Mahmoodi |
1. Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran 2. Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran

Fatemeh Karami |
1.Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran 2.Department of Medical Genetics, Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran

Hamidreza Abdollahi |
Department of Medical Genetics, Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran

Navidreza Giahi |
Akhtar Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Kouros Divsalar |
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

Amin Honarmand |
Department of Emergency Medicine, Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, Iran

Mohammad Hossein Modarressi |
Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran

نشانی اینترنتی https://ahj.kmu.ac.ir/article_92216_4f3a5d2cc8fd72f41d5142ac91eb0527.pdf
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