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Iranian Journal of Basic Medical Sciences، جلد ۲۶، شماره ۱۰، صفحات ۱۲۰۲-۱۲۱۱
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عنوان انگلیسی Deregulation of apoptotic proteins by induction of Dendropthae falcata (L.f.) Ettingsh plant extract in breast cancer cells: A proteome-wide analysis
چکیده انگلیسی مقاله Objective(s): The present study evaluated the protein-based analysis to unravel the role and mechanism behind the Dendropthae falcata plant extract treatment in breast cancer cells. Materials and Methods: The protein sample was extracted from the cancer cells after treatment with the plant extract and subjected to two-dimensional electrophoresis for protein separation. Further, the proteins that were differentially regulated among the samples which were treated and non-treated were selected and processed further for protein identification using a tandem mass spectrometry approach.Results: Using these strategies, we identified 16 potential candidates which were showing remarkable changes in treated samples. All the candidates were analyzed further for gene ontology analysis, and it was observed that all proteins were involved in multiple pathways pertaining to the carcinogenesis process. Specifically, apoptotic pathway proteins including BAD, BIK, BID, CASP8, MCL1, BCL2, and BAK1 were highly impacted by treatment with D. falcata plant extract. All these protein hits were further taken for validation experiments using RT PCR analysis. Conclusion: Initiation of these apoptotic proteins by D. falcata plant extract treatment in breast cancer cells shows a positive direction toward nature-based alternative medicine.
کلیدواژه‌های انگلیسی مقاله 2D-Gel Electrophoresis, Breast cancer cell line, Dendropthae falcate, Mass spectrometry, Medicinal plant, Proteomics
نویسندگان مقاله | Alwin Beschi Durairaj
Department of Botany & Research Center, Scott Christian College, Nagercoil – 629003, Affiliated to Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli – 627012, Tamil Nadu, India


| Ananthi Sivaganam
Department of Proteomics, Clinbiocare Technology, Tenkasi – 627 814, Tamil Nadu, India|Centre of Molecular Medicine and Diagnostics (COMManD), Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India


| Reginald Appavoo Monikam
Department of Botany & Research Center, Scott Christian College, Nagercoil – 629003, Affiliated to Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli – 627012, Tamil Nadu, India


| Rajapandiyan Krishnamoorthy
Department of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia


| Mohammad Z. Ahmed
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia


| Alqahtani Ali S.
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia


| Ponani Kaja Mideen
Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi, USA
نشانی اینترنتی https://ijbms.mums.ac.ir/article_22552.html
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نوع مقاله منتشر شده Original Article
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