این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Acta Medica Iranica، جلد ۶۱، شماره ۵، صفحات ۲۶۴-۲۶۹
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Abnormal Promoter Methylation of Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2) Gene in the Pathogenesis of Crohn’s Disease
چکیده انگلیسی مقاله Changes in the expression of nucleotide-binding oligomerization domain containing 2 (NOD2) play an important role in the pathogenesis of several autoimmune diseases, such as inflammatory bowel diseases (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC). It seems that epigenetic modifications, including DNA methylation, have an important role in the suppression of gene activity. In this study, the relationship between DNA methylation patterns of the promoter region of the NOD2 gene and the pathogenesis of CD was assessed. Colonic mucosal samples were obtained from 15 Iranian patients with CD and 15 matched healthy controls with no history of autoimmune diseases. After bisulfite conversion of genomic DNA, the DNA methylation status of three CpG sites in the promoter region of the NOD2 gene was determined by the real-time quantitative multiplex methylation-specific PCR assay. Using this approach, we identified a decreased level of methylation of the NOD2 promoter in the colonic mucosa of patients with CD (0.128±0.093 vs. 0.025±0.016, unmethylated DNA in CD vs. healthy controls, respectively, P< 0.000). According to our findings, promoter hypomethylation of the NOD2 gene in the colonic mucosa might contribute to the development and severity of CD.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Golshid Sanati
Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran


| Mehrdad Noruzinia
Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran


| Davood Jafari
Department of Immunology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran


| Mohammad Ahmadvand
Department of Medical Genetics, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran


| Shahram Teimourian
Department of Genetics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran


| Naser Ebrahimi Daryani
Department of Internal Medicine, Division of Gastroenterology, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran


| Nima Rezaei


نشانی اینترنتی https://acta.tums.ac.ir/index.php/acta/article/view/9867
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات