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Molecular Biology Research Communications، جلد ۱۲، شماره ۴، صفحات ۱۴۹-۱۵۴
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عنوان انگلیسی Human BKV large T genome detection in prostate cancer and benign prostatic hyperplasia tissue samples by nested PCR: A case-control study
چکیده انگلیسی مقاله Human BK polyomavirus (BKPyV) is a latent infectious agent in the genitourinary tract associated with hemorrhagic cystitis and nephropathy. This virus can be a risk factor for various human malignancies, including prostate cancer (PCa). It may contribute to prostate cancer development, as it demonstrates oncogenic properties by encoding oncoproteins. This study assessed the prevalence of this virus in benign and malignant prostate tissues. Between 2009 and 2019, 49 formalin-fixed paraffin-embedded (FFPE) PCa and 49 benign prostatic hyperplasia (BPH) samples were gathered from the pathology department of a tertiary care university hospital. They were used as cases and controls, respectively. After deparaffinization and DNA extraction, nested PCR was applied to identify the BKPyVgp5 gene (LTAg) using inner and outer primers. The nested PCR showed a 278-bp bond corresponding to the BKPyVgp5 genome (LTAg) in 53.1% (26/49) of PCa and 14.3% (7/49) of BPH (p< 0.001). The presence of BKV was significantly associated with an increased risk of PCa development (OR=6.78, 95% CI=2.55–18.02, p< 0.001). The BKV LTAg gene was significantly more prevalent in PCa samples than in BPH samples. These results demonstrate the presence of the virus in prostate cancer tissues.
کلیدواژه‌های انگلیسی مقاله BK Virus, Prostate Cancer, Benign Prostatic Hyperplasia, nested PCR
نویسندگان مقاله Narges Tavassoli |
Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Arastoo Vojdani |
Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Sara Salimi-Namin |
Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Majid Khadem-Rezaiyan |
Department of Community Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Mahmoudreza Kalantari |
Department of Pathology, Mashhad Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Masoud Youssefi |
Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

نشانی اینترنتی https://mbrc.shirazu.ac.ir/article_7206_4c0d7640e68116f0bac4418baf08df17.pdf
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