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Iranian Journal of Pediatric Hematology and Oncology، جلد ۱۳، شماره ۴، صفحات ۲۴۱-۲۵۰
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عنوان انگلیسی Parvovirus B19 Infection May Potentially Determine the Fate of Hematopoiesis by Altering the Human Bone Marrow Mesenchymal Stem Cells Differentiation
چکیده انگلیسی مقاله Background: Human bone marrow mesenchymal stem cells (hBM-MSCs), as supporters for hematopoiesis, differentiate into osteoblasts and adipocytes. Studies showed that infection of hBM-MSCs by Parvovirus B19 (B19V) can affect the differentiation capability of hBM-MSCs. This study aims to evaluate B19V effects on the differentiation of hBM-MSCs. Materials and Methods: In this experimental study hBM-MSCs were cultured up to passage 3. Nucleofection was subsequently employed to deliver a plasmid containing the B19V genome into the cells. The transfected cells were then differentiated into osteoblast and adipocyte lineages. qRT-PCR was then performed to analyze the differentiation 14 days after transfection. Results: On the 14th day after induction the findings demonstrated a significant increase in adipocyte-specific (PPARγ and LPL) gene expression compared to the control group (p< 0.05) and a slight but not statistically significant decrease in the expression of the osteocyte-specific genes (RUNX2 and osteocalcin) (p>0.05). Conclusion: The results suggest that B19V infection can promote the differentiation of hBM-MSCs towards adipocytes and affect the bone marrow microenvironment as well as hematopoiesis.
کلیدواژه‌های انگلیسی مقاله Adipocyte, Bone marrow, Mesenchymal stem cell, Osteoblast, Parvovirus B19
نویسندگان مقاله | Azin Elmi
Department of Medical Biotechnology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran


| Amir Atashi
Stem Cells and Tissue Engineering Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.


| Nematollah Gheibi
Department of Medical Biotechnology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.


| Shahin Amiri
Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran


| Monireh Ajami
Department of Hematology, Faculty of Paramedical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran


| Mansoureh Ajami
Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, Iran


| Razieh Mohammadihaji
Department of Medical Biotechnology, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.


| Naeimeh Khodabandeloo
Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.


| Mehdi Azad
Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
نشانی اینترنتی http://ijpho.ssu.ac.ir/browse.php?a_code=A-10-911-1&slc_lang=en&sid=1
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