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Iranian Journal of Basic Medical Sciences، جلد ۲۶، شماره ۱۲، صفحات ۱۳۹۰-۱۳۹۹
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عنوان انگلیسی Short-hairpin RNA-mediated suppression of cortactin may inhibit the migration and invasion abilities of endometrial cancer cells by reducing lamellipodia
چکیده انگلیسی مقاله Objective(s): The prognosis of endometrial cancer (EC) is significantly affected by tumor infiltration and metastasis. Cortactin (CTTN) regulates infiltration and metastasis in other tumors. Studies on the role and mechanism of CTTN in EC are limited and further studies are needed.Materials and Methods: Quantitative PCR and immunohistochemistry were used to detect Ras-associated C3 botulinum toxin substrate 1 (Rac1) and CTTN in EC and normal tissues. The relationship between the expression of these two genes and their prognostic factors was analyzed. A CTTN-RNAi lentiviral system was constructed and transfected into EC cells. Migration and invasion were evaluated by scratch assay, transwell migration, and invasion assays. Pseudopodia formation was observed by immunofluorescence staining. Western blotting was performed to detect the expression of Rac1.Results: The expression levels of Rac1 and CTTN in EC tissues were significantly higher than those in normal tissues. In the EC group, Rac1 and CTTN levels were correlated. The protein expression levels of Rac1 and CTTN were related to myometrial invasion and stage. After CTTN knockdown, the migration rate, invasiveness, and migratory ability of EC cells decreased significantly. Lamellipodia was observed to disappear with the appearance of blebs. Rac1 protein expression was decreased after CTTN knockdown.Conclusion: CTTN may promote the invasion and migration of EC by lamellipodia. This effect may be related to the regulation of Rac1 by CTTN.
کلیدواژه‌های انگلیسی مقاله Cell migration, Cortactin, Endometrial neoplasms, Pseudopodia, Rac1
نویسندگان مقاله | Huisi Lin
Department of Gynecology and Obstetrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, China


| Yujuan Fan
Department of Gynecology and Obstetrics, University of the Chinese Academy of Sciences, Shenzhen Hospital, Shenzhen, China


| Zhifu Zhi
Department of Gynecology and Obstetrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, China


| Lihong Pang
Department of Gynecology and Obstetrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, China|Key Laboratory of Early Prevention and Treatment for Regional High-Frequency Tumor (Guangxi Medical University), Ministry of Education, Nanning, China


| Dan Sun
Department of Gynecology and Obstetrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, China
نشانی اینترنتی https://ijbms.mums.ac.ir/article_22790.html
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