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Iranian Journal of Basic Medical Sciences، جلد ۲۶، شماره ۱۲، صفحات ۱۴۲۳-۱۴۳۰
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عنوان انگلیسی Considering the protective effect of exendin-4 against oxidative stress in spiral ganglion neurons
چکیده انگلیسی مقاله Objective(s): The protection of spiral ganglion neurons (SGNs) is crucial for hearing loss. Exendin-4 has been shown to have neuroprotective effects in several neurological disorders. Therefore, this study aimed to investigate the effect of the glucagon-like protein-1 receptor (GLP-1R) agonist exendin-4 on kanamycin-induced injury in mouse SGNs in vitro.Materials and Methods: In this study, GLP-1R expression in SGNs was verified by immunofluorescence and immunohistochemical staining. In vitro-cultured SGNs and the organ of Corti were exposed to kanamycin with or without exendin-4 treatment. The cell survival rate was measured using the cell counting kit-8 assay, and the damage to auditory nerve fibers (ANF) projecting radially from the SGNs was evaluated using immunofluorescence staining. Reactive oxygen species (ROS) content was determined by flow cytometry, and glutathione peroxidase (GSH-Px) content, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) content were determined by spectrophotometry. Protein expression of nuclear factor erythroid-2-related factor 2/heme oxygenase-1 (Nrf2/HO-1) was detected using western blotting.Results: GLP-1R was expressed in SGNs. Treatment with 1 mM kanamycin for 24 hr induced SGN damage. Exendin-4 (100 nM) had a protective effect against kanamycin-induced SGN cell injury, improved cell survival rate, reduced nerve fiber injury, increased SOD activity and GSH-Px level, and reduced MDA and ROS contents. The Nrf2/HO-1 pathway was activated.Conclusion: Exendin-4 alleviates oxidative damage and exerts neuroprotective effects in kanamycin-induced SGN injury through the Nrf2/HO-1 signaling pathway. Exendin-4 has the potential to prevent or treat hearing loss due to SGN damage.
کلیدواژه‌های انگلیسی مقاله Exenatide, Hearing Loss, Kanamycin, Mice, Oxidative Damage, Spiral ganglio
نویسندگان مقاله | Hongxia Wu
Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical Chongqing, China


| Yangxi Ou
Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical Chongqing, China


| Siji Wang
Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical Chongqing, China


| Fenghui Yu
Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical Chongqing, China


| Xiaoxia Fan
Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical Chongqing, China


| Houyong Kang
Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical Chongqing, China


| Tao Chen
Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical Chongqing, China
نشانی اینترنتی https://ijbms.mums.ac.ir/article_22887.html
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