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Cell Journal، جلد ۲۴، شماره ۱۲، صفحات ۷۴۸-۷۵۶
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عنوان انگلیسی Impact of Intraventricular Human Adipose-Derived Stem Cells Transplantation with Pregnenolone Treatment on Remyelination of Corpus Callosum in A Rat Model of Multiple Sclerosis
چکیده انگلیسی مقاله Objective
Multiple sclerosis (MS) is known as a nerve tissue disorder, which causes demyelination of central nervous
system (CNS) fibers. Cell-based treatment is a novel strategy for the treatment of demyelinating diseases such as
MS. Adipose-derived stem cells (ADSCs) have neuroprotective and neuroregenerative effects and pregnenolone as a
neurosteroid has remarkable roles in neurogenesis. We intend to examine the impact of intraventricular transplantation
of human ADSCs and systemic injection of pregnenolone on the remyelination of a rat model cuprizone-induced
demyelination.
Materials and Methods
This experimental study was performed on 36 male Wistar rats that received a regular diet
and a cuprizone diet for 3 weeks for M.S. induction. Through lipoaspirate surgery, human-ADSCs (hADSCs) were
obtained from a patient. Six groups of rats (n=6): healthy, MS, sham, pregnenolone injection, ADSCs transplantation,
and pregnenolone injection/ADSCs transplantation were included in this study. For assessment of remyelination,
transmission electron microscopy (TEM), immunohistochemistry staining, real-time reverse transcription-polymerase
chain reaction (RT-PCR), and enzyme-linked immunosorbent assay (ELISA) were performed.

Results
TEM outcomes revealed an increase in the thickness of the fibers myelin in the treatment groups (P<0.05). We
also observed a significant upregulation of MBP, PDGFR-α, and MOG after treatment with hADSCs and pregnenolone
compared to other study groups (P<0.001). These results were confirmed by immunostaining analysis. Moreover, there
was no significant difference between the ADSCs/pregnenolone group and the control group regarding the level of
MBP, A2B5, and MOG proteins in ELISA.

Conclusion
Our data implied that the remyelination and cell recovery were more improved by intraventricular ADSCs
transplantation and pregnenolone injection after inducing a rat model of MS.
کلیدواژه‌های انگلیسی مقاله Adipose-Derived Stem Cells, intraventricular, multiple sclerosis, Pregnenolone
نویسندگان مقاله Mohammad Mardani |
Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Rasoul Ganji |
Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Nazem Ghasemi |
Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Mohammad Kazemi |
Department of Genetics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Shahnaz Razavi |
Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

نشانی اینترنتی https://www.celljournal.org/article_253345_b7a3f9fd03854243926dd8b750854c29.pdf
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