این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۲۴، شماره ۱۱، صفحات ۶۳۷-۶۴۶
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Assessment of Cytotoxicity and Odontogenic/Osteogenic Differentiation Potential of Nano-Dentine Cement Against Stem Cells from Apical Papilla
چکیده انگلیسی مقاله Objective

Assessment of the cytotoxicity of novel calcium silicate-based cement is imperative in endodontics. This
experimental study aimed to assess the cytotoxicity and odontogenic/osteogenic differentiation potential of a new
calcium silicate/pectin cement called Nano-dentine against stem cells from the apical papilla (SCAPs).


Materials and Methods

In this experimental study, the cement powder was synthesized by the sol-gel technique.
Zirconium oxide was added as opacifier and Pectin, a plant-based polymer, and calcium chloride as the liquid to
prepare the nano-based dental cement. Thirty-six root canal dentin blocks of human extracted single-canal premolars
with 2 mm height, flared with #1, 2 and 3 Gates-Glidden drills were used to prepare the cement specimens. The
cement, namely mineral trioxide aggregate (MTA), Biodentine, and the Nano-dentine were mixed according to the
manufacturers’ instructions and applied to the roots of canal dentin blocks. The cytotoxicity and odontogenic/osteogenic
potential of the cement were evaluated by using SCAPs.



Results

SCAPs were characterized by the expression of routine mesenchymal cell markers and differentiation
potential to adipocytes, osteoblasts, and chondrocytes. Cement displayed no significant differences in cytotoxicity or
calcified nodules formation. Gene expression analysis showed that all three types of cement induced significant downregulation
of COLA1; however, the new cement induced significant up-regulation of RUNX2 and SPP1 compared to
the control group and MTA. The new cement also induced significant up-regulation of TGFB1 and inducible nitric oxide
synthase (iNOS) compared with Biodentine and MTA.


Conclusion

The new Nano-dentin cement has higher odontogenic/osteogenic potential compared to Biodentine and
MTA for differentiation of SCAPs to adipocytes, osteoblasts, and chondrocytes.
کلیدواژه‌های انگلیسی مقاله Biodentine, Calcium Silicate, Mineral Trioxide Aggregate, Stem cells
نویسندگان مقاله Eshagh Ali Saberi |
Department of Endodontics, Faculty of Dentistry, Oral and Dental Diseases Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

Narges Farhad Mollashahi |
Department of Endodontics, Faculty of Dentistry, Oral and Dental Diseases Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

Fatemeh Ejeian |
Department of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran

Marzieh Nematollahi |
Department of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran

Omolbanin Shahraki |
Pharmacology Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

Arezou Pirhaji |
Department of Endodontics, Faculty of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran.

Mohammad Hossein Nasr Esfahani |
Department of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran

نشانی اینترنتی https://www.celljournal.org/article_253341_158c7ebd814cabc4ff59623a62b95c79.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات