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Cell Journal، جلد ۲۳، شماره ۱، صفحات ۶۱-۶۹
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Elevated Secretion of Aldosterone Increases TG/HDL-C Ratio and Potentiates The Ox-LDL-Induced Dysfunction of HUVEC
چکیده انگلیسی مقاله Objective
Atherosclerosis (AS) is one of the most common causes of human death and disability. This study is designed to investigate the roles of aldosterone (Aldo) and oxidized low-density lipoprotein (Ox-LDL) in this disease by clinical data and cell model.
Materials and Methods
In this experimental study, clinical data were collected to investigate the Aldo role for the patients with primary aldosteronism or adrenal tumors. Cell viability assay, fluorescence-activated cell sorting (FACS) assay, apoptosis assay, cell aging analysis, and matrigel tube formation assay were performed to detect effects on human umbilical vein endothelial cells (HUVECs) treated with Aldo and/or Ox-LDL. Quantitative polymerase chain reaction (qPCR) and Western blot analysis were performed to figure out critical genes in the process of endothelial cells dysfunction induced by Aldo and/or Ox-LDL.
Results
We found that the Aldo level had a positive correlation with the TG/HDL-C ratio. Endothelial cell growth, angiogenesis, senescence, and apoptosis were significantly affected, and eNOS/Sirt1, the value of Bcl-2/Bax and Angiopoietin1/2 were significantly affected when cells were co-treated by Aldo and Ox-LDL.
Conclusion
Elevated Aldo with high Ox-LDL together may accelerate the dysfunction of HUVEC, and the Ox-LDL, especially for those patients with high Aldo should be well controlled. The assessment of the role of Aldo may provide a theoretical basis for the effective prevention and investigation of a new treatment of AS.
کلیدواژه‌های انگلیسی مقاله Aldosterone, Atherosclerosis, Human Umbilical Vein Endothelial Cells, Triglyceride/High, Density Lipoprotein Cholesterol, Oxidized Low, Density Lipoprotein
نویسندگان مقاله Qian Zhang |
.Department of Cardiovascular Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China

Yiwen Pan |
.Department of Cell Biology, School of Life Sciences, Central South University, Changsha, Hunan, China

Xiaochun Ma |
.Department of Cardiovascular Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China

Hao Yang |
.Department of Cell Biology, School of Life Sciences, Central South University, Changsha, Hunan, China

Jun Chang |
.Department of Cell Biology, School of Life Sciences, Central South University, Changsha, Hunan, China

Ling Hong |
.Department of Cell Biology, School of Life Sciences, Central South University, Changsha, Hunan, China

نشانی اینترنتی https://www.celljournal.org/article_250690_2aecfcb0e263a2299063bee969a5bc3f.pdf
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