این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۲۲، شماره ۴، صفحات ۴۰۶-۴۱۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Enhancement of The Stability of Human Growth Hormone by Using Tris(hydroxymethyl)aminomethane: Molecular Docking and Experimental Analysis
چکیده انگلیسی مقاله Objective
It is so difficult to formulate human growth hormone (hGH) in a solution with high stability and new drug delivery system (NDDs) due to physiochemical instability. The purpose of this study was to investigate the possibility of using Tris as a hGH stabilizer.
Materials and Methods
In this experimental study, the role of tris(hydroxymethyl)aminomethane (Tris) was evaluated as a hGH stabilizing agent in phosphate buffer, as a practical aqueous solution and a media to release NDDs. High- performance liquid chromatography (HPLC) and enzyme-linked immune sorbent assay (ELISA) were applied to investigate the stability of hGH in solutions and dynamic light scattering (DLS) was used to measure the effect of Tris on the hydrodynamic size of hGH in aqueous solutions. Ultra violet (UV) spectrophotometry was used to check the hGH spectrum. In computational study, formation of ligand-protein complex of the Tris-hGH, and the intermolecular interactions between Tris and hGH were studied by molecular docking modeling.
Results
The results demonstrated that Tris at the optimum concentration, increases hGH stability in aqueous solutions. Also, molecular docking modeling confirmed that amino acid residues such as tyrosine (Tyr), proline (Pro), glutamic acid (Glu), aspartic acid (Asp), leucine (Leu), and phenylalanine (Phe) in hGH structure, were linked with Tris as a ligand.
Conclusion
It seems that interactions between hGH and Tris are the most important reason for increment of the physicochemical stability of hGH in aqueous solutions containing Tris.
کلیدواژه‌های انگلیسی مقاله Human growth hormone, Molecular modeling, Protein stability, Tris(hydroxymethyl)aminomethane
نویسندگان مقاله Siyavash Mirzaei |
.Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

Hamid Mobedi |
.Department of Novel Drug Delivery Systems, Iran Polymer and Petrochemical Institute, Tehran, Iran

Hamid Gourabi |
.Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

Mohammad Hosein Sanati |
.Medical Genetics Department, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

Sakine Khezli |
.Department of Novel Drug Delivery Systems, Iran Polymer and Petrochemical Institute, Tehran, Iran

Hamid Omidian |
4.Department of Pharmaceutical Sciences, Nova Southeastern University, Pharmaceutical Sciences, Davie, Florida, USA

Masoume Ighaeie |
5.Computational Nano Physical Chemistry Laboratory, Department of Chemistry, Azerbaijan Shahid Madani University, Tabriz, Iran

نشانی اینترنتی https://www.celljournal.org/article_250664_b79290bc7f34016c440544e442982cb2.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات