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Cell Journal، جلد ۲۲، شماره ۴، صفحات ۴۸۲-۴۹۰
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عنوان انگلیسی Methylation Status of MTHFR Promoter and Oligozoospermia Risk: An Epigenetic Study and in Silico Analysis
چکیده انگلیسی مقاله Objective
In this study, we evaluated the effects of promoter methylation of MTHFR on oligozoospermia risk, followed by an in silico analysis.
Materials and Methods
In a case-control study, semen samples were collected from infertile and healthy control men. MTHFR promoter region was amplified by methylation-specific polymerase chain reaction (PCR). Finally, the promoter region of MTHFR was analyzed by bioinformatics software.
Results
Our data revealed significant associations of CpG island promoter methylation with oligozoospermia in a case-control study. In silico analysis showed that promoter contains a strong nucleosome exclusion region, a bonafide CGIs, six PROSITE motifs without a defined TATA box and 14 transcription factor (TF) binding sites, which are directly involved in spermatogenesis
Conclusion
Based on our findings, methylation of the MTHFR gene promoter region may be a risk factor for oligozoospermia. However, this is a preliminary report representing data for future comprehensive studies to make a clinical conclusion on the potential biomarker role of methylation of this promoter in elevating susceptibility to oligozoospermia.
کلیدواژه‌های انگلیسی مقاله Bioinformatics, DNA methylation, Male infertility, Methylenetetrahydrofolate reductase, Oligozoospermia
نویسندگان مقاله Atefeh Rezaeian |
Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran; Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar,

Mohammad Karimian |
Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran

نشانی اینترنتی https://www.celljournal.org/article_250673_1a917b85250306ebd14c85385aae3904.pdf
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