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Cell Journal، جلد ۲۲، شماره ۳، صفحات ۲۸۳-۲۹۲
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عنوان انگلیسی Kinetics of Oncolytic Reovirus T3D Replication and Growth Pattern in Mesenchymal Stem Cells
چکیده انگلیسی مقاله Objective
Currently, application of oncolytic-virus in cancer treatment of clinical trials are growing. Oncolytic-reovirus is an attractive anti-cancer therapeutic agent for clinical testing. Many studies used mesenchymal stem cells (MSCs) as a carrier cell to enhance the delivery and quality of treatment with oncolytic-virotherapy. But, biosynthetic capacity and behavior of cells in response to viral infections are different. The infecting process of reoviruses takes from two-hours to one-week, depends on host cell and the duration of different stages of virus replication cycle. The latter includes the binding of virus particle, entry, uncoating, assembly and release of progeny-viruses. We evaluated the timing and infection cycle of reovirus type-3 strain Dearing (T3D), using one-step replication experiment by molecular and conventional methods in MSCs and L929 cell as control.
Materials and Methods
In this experimental study, L929 and adipose-derived MSCs were infected with different multiplicities of infection (MOI) of reovirus T3D. At different time points, the quantity of progeny viruses has been measured using virus titration assay and quantitative real-time polymerase chain reaction (qRT-PCR) to investigate the ability of these cells to support the reovirus replication. One-step growth cycle were examined by 50% cell culture infectious dose (CCID50) and qRT-PCR.
Results
The growth curve of reovirus in cells shows that MOI: 1 might be optimal for virus production compared to higher and lower MOIs. The maximum quantity of virus production using MOI: 1 was achieved at 48-hours post-infection. The infectious virus titer became stationary at 72-hours post-infection and then gradually decreased. The virus cytopathic effect was obvious in MSCs and this cells were susceptible to reovirus infection and support the virus replication.
Conclusion
Our data highlights the timing schedule for reovirus replication, kinetics models and burst size. Further investigation is recommended to better understanding of the challenges and opportunities, for using MSCs loaded with reovirus in cancer-therapy.
کلیدواژه‌های انگلیسی مقاله Cancer, Mesenchymal stem cells, Oncolytic Viruses, Quantitative Real, Time Polymerase Chain Reaction, Reovirus Type 3
نویسندگان مقاله Razieh Sadat Banijamali |
.Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Hoorieh Soleimanjahi |
.Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Sara Soudi |
.Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Hesam Karimi |
.Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Asghar Abdoli |
.Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran

Seyed Mahmood Seyed Khorrami |
.Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Keivan Zandi |
4.Center for AIDS Research, Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia, USA

نشانی اینترنتی https://www.celljournal.org/article_250649_3615ea293f412499efc1c6e5fbcfef45.pdf
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