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Cell Journal، جلد ۲۲، شماره ۳، صفحات ۳۲۵-۳۳۳
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عنوان انگلیسی PolyI:C Upregulated CCR5 and Promoted THP-1-Derived Macrophage Chemotaxis via TLR3/JMJD1A Signalling
چکیده انگلیسی مقاله Objective
This study aimed to evaluate the specific roles of polyinosinic:polycytidylic acid (polyI:C) in macrophage chemotaxis and reveal the potential regulatory mechanisms related to chemokine receptor 5 (CCR5).
Materials and Methods
In this experimental study, THP-1-derived macrophages (THP1-Mφs) induced from THP- 1 monocytes were treated with 25 μg/mL polyI:C. Toll-like receptor 3 (TLR3), Jumonji domain-containing protein (JMJD)1A, and JMJD1C small interfering RNA (siRNAs) were transfected into THP1-Mφs. Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) was used to detect the expression levels of TLR3, CCR5, 23 Jumonji C domain-containing histone demethylase family members, JMJD1A, and JMJD1C in THP1-Mφs with different siRNAs transfections. Western blot was performed to detect JMJD1A, JMJD1C, H3K9me2, and H3K9me3 expressions. A transwell migration assay was conducted to detect THP1-Mφ chemotaxis toward chemokine ligand 3 (CCL3). A chromatin immunoprecipitation (ChIP) assay was performed to detect H3K9me2-CCR5 complexes in THP1- Mφs.
Results
PolyI:C significantly upregulated CCR5 in THP1-Mφs and promoted chemotaxis toward CCL3 (P < 0.05); these effects were significantly inhibited by TLR3 siRNA (P < 0.01). JMJD1A and JMJD1C expression was significantly upregulated in polyI:C-stimulated THP1-Mφs, while only JMJD1A siRNA decreased CCR5 expression (P < 0.05). JMJD1A siRNA significantly increased H3K9me2 expression in THP1-Mφs but not in polyI:C-stimulated THP1-Mφs. The ChIP result revealed that polyI:C significantly downregulated H3K9me2 in the promoter region of CCR5 in THP1- Mφs.
Conclusion
PolyI:C can enhance THP1-Mφ chemotaxis toward CCL3 regulated by TLR3/JMJD1A signalling and activate CCR5 expression by reducing H3K9me2 in the promoter region of CCR5.
کلیدواژه‌های انگلیسی مقاله Chemokine Receptor 5, Chemotaxis, macrophages, Polyinosinicpolycytidylic Acid
نویسندگان مقاله Xiaoxiao Yu |
.Department of Paediatrics, Qilu Hospital of Shandong University, Jinan, Shandong, China

Huayang Wang |
. Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, Shandong, China

Hongjia Shao |
.Department of Paediatrics, Qilu Hospital of Shandong University, Jinan, Shandong, China

Cuijuan Zhang |
.Department of Pathology, Qilu Hospital of Shandong University, Jinan, Shandong, China

Xiuli Ju |
.Department of Paediatrics, Qilu Hospital of Shandong University, Jinan, Shandong, China

Jie Yang |
.Department of Paediatrics, Qilu Hospital of Shandong University, Jinan, Shandong, China

نشانی اینترنتی https://www.celljournal.org/article_250654_61f069e8b1922521ed28fbdd30de000f.pdf
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