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Cell Journal، جلد ۲۲، شماره ۲، صفحات ۱۷۸-۱۸۴
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عنوان انگلیسی A Comparative Study of HOTAIR Expression in Breast Cancer Patient Tissues and Cell Lines
چکیده انگلیسی مقاله Objective
Recent data suggest that increased levels of the HOTAIR long non-coding RNA (lncRNA) are involved in the development of various types of malignancy, including breast cancer. The aim of present study was to investigate HOTAIR lncRNA expression profile in breast cancer (BC) patients and cell lines.
Materials and Methods
In this experimental study, expression level of HOTAIR lncRNA was evaluated in BC and normal tissues of 15 patients as well as MDA-MB-231, MCF-7 and MCF-10A cell lines, using quantitative reverse- transcription polymerase chain reaction (qRT-PCR). HOTAIR lncRNA expression levels were estimated using 2-ΔΔCt method. Further, receiver operating characteristic (ROC) curve analysis was done to evaluate the selected lncRNA diagnostic potential. The Cox’s proportional hazards regression model was performed to evaluate the predictive value of this lncRNA level in BC patients.
Results
The results of present study demonstrated no significant difference in the expression of HOTAIR lncRNA in MCF7 and MDA-MB-231 cancer cell lines compared to MCF-10A as normal cell line (P > 0.05). However, we observed a significantly increase in the expression of HOTAIR in BC patients compared to normal tissues (P < 0.001). Significant associations were found between gene expression and tumour size and margin. We found 91.1% sensitivity and 95.7% specificity of circulating HOTAIR with an area under the ROC curve of 0.969. The Kaplan-Meier analysis indicated significant correlation between HOTAIR expression and overall survival.
Conclusion
This study demonstrated that expression of HOTAIR is increased in BC and might be associated with its progression. According to these findings, HOTAIR expression could be proposed as biomarkers for BC early diagnosis and prognosis.
کلیدواژه‌های انگلیسی مقاله Breast cancer, Cell line, lncRNA, Quantitative Reverse, Transcription Polymerase Chain Reaction
نویسندگان مقاله Asghar Arshi |
.Young Researchers and Elite Club, Najafabad Branch, Islamic Azad University, Najafabad, Iran

Farzaneh Raeisi |
.Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran ;.Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Esmaeil Mahmoudi |
.Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Fatemeh Mohajerani |
4.Department of Genetics, Faculty of Modern Medical Science, Islamic Azad University of Medical Sciences of Tehran, Tehran, Iran

Hamidreza Kabiri |
.Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Razieh Fazel |
5.Student Research Committee, Jahrom University of Medical Sciences, Jahrom, Iran

Maedeh Zabihian-Langeroudi |
6.Department of Biology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran

Amela Jusic |
7.Department of Biology, Faculty of Natural Sciences and Mathematics, University of Tuzla, Tuzla, Bosnia and Herzegovina

نشانی اینترنتی https://www.celljournal.org/article_250638_9cf64f1b84405906a5491886baceae82.pdf
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