این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۲۲، شماره ۱، صفحات ۱-۸
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی The Effect of Radiation Emitted by Cell Phone on The Gelatinolytic Activity of Matrix Metalloproteinase-2 and -9 of Mouse Pre-Antral Follicles during In Vitro Culture
چکیده انگلیسی مقاله Objective
The unfavorable effects of electromagnetic radiation (EMR) emitted by the cell phone on reproduction health are controversial. Metalloproteinases play a vital role in ovarian follicle development. This study was designed to investigate the effects of exposure to the cell phone on the gelatinolytic activity of in vitro cultured mouse pre-antral follicle.
Materials and Methods
In this experimental study, pre-antral follicles were isolated from ovaries of immature mice (n=16) and cultured with or without exposure to the cell phone in talking mode for 60 minutes. The gelatinolytic activity was evaluated through the zymography method, as well as the gene expression of matrix metalloproteinases (MMPs) namely MMP-2 and -9 and tissue inhibitors of metalloproteinases (TIMPs) namely, TIMP-1 and -2 by the real-time polymerase chain reaction (PCR) method. Also, in parallel, the development of pre-antral follicles was assessed.
Results
The maturation parameters of the cell phone-exposed pre-antral follicles were significantly lower compared with the control group (P < 0.05). The gelatinolytic activity was significantly decreased in the cell phone-exposed pre- antral follicles compared with the control group (P < 0.05). The relative mRNA expression of the MMP-2 gene was significantly (P < 0.05) increased in the cell phone-exposed pre-antral follicles whereas the expression rate of the MMP-9 gene was considerably (P < 0.05) reduced when compared with the control group. Conversely, the relative expression of the TIMP-1 was markedly (P < 0.05) increased in the cell phone-exposed pre-antral follicles while the expression of the TIMP-2 was (P < 0.05) significantly diminished in comparison with the control group.
Conclusion
Exposure to the cell phone alters the growth and maturation rate of murine ovarian follicle through the changing in the expression of the MMP-2 and -9 genes, as well as the gelatinolytic activity.
کلیدواژه‌های انگلیسی مقاله Cell phone, Gelatinase, Ovarian follicles, Radiation
نویسندگان مقاله Fariba Azimipour |
School of Biology, Damghan University, Damghan, Iran

Saeed Zavareh |
School of Biology, Damghan University, Damghan, Iran;Institute of Biological Sciences, Damghan University, Damghan, Iran

Taghi Lashkarbolouki |
School of Biology, Damghan University, Damghan, Iran;Institute of Biological Sciences, Damghan University, Damghan, Iran

نشانی اینترنتی https://www.celljournal.org/article_250614_e12ca23fb9a59a5d7262da11ea86cfd9.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات