این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۲۲، شماره ۱، صفحات ۱۷-۲۲
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Elevated Expression of Cytosolic Phospholipase A2Delta Is Associated with Lipid Metabolism Dysregulation during Hepatocellular Carcinoma Progression
چکیده انگلیسی مقاله Objective
Liver cancer is the third rank amongst the common malignancies, causing maximum death in the patients diagnosed with cancers. Currently available biomarkers are not enough sensitive for early diagnosis of hepatocellular carcinoma (HCC). This makes difficult management of HCC. With the aim of finding new generation of proteomic-based biomarkers, the represented study was designed to characterize the differentially expressed proteins at different stages of HCC initiation and at progression. This could lead to find potential biomarkers for early detection of HCC.
Materials and Methods
In this experimental study, we report induction of HCC by administrating chemical carcinogens in male Wistar rats. Disease progression was monitored by histological evaluation. Serum proteomic analyses such as 2 dimensional (2D)-electrophoresis, MALDI-TOF-MS/MS and Western blot have been used to analyze and characterize the differentially expressed proteins during HCC development.
Results
HCC initiation and tumorigenesis were observed at one and four months post carcinogen treatment, respectively. One of the differentially-expressed proteins, namely, cytosolic phospholipase A2delta was significantly up-regulated at very early stage of HCC development. Its expression continued to increase during cancer progression and hepatotumorigenesis stages. Its elevated expression has been confirmed by Western blot analysis. Consistent to this, analyses of the sera in the clinically confirmed liver cancer patients showed elevated expression of this protein, further validating our experimental results.
Conclusion
This study suggests that elevation in the expression of cytosolic phospholipase A2delta is associated with progression of HCC.
کلیدواژه‌های انگلیسی مقاله Chemical Carcinogens, Cytosolic Phospholipase A2Delta, Hepatocellular Carcinoma, MALDI, TOF, MS/MS, Western Blot Analysis
نویسندگان مقاله Maryam Ranjpour |
Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, India

Saima Wajid |
Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, India

Swatantra Kumar Jain |
Department of Medical Biochemistry, HIMSR, Jamia Hamdard, New Delhi, India

نشانی اینترنتی https://www.celljournal.org/article_250616_d137d90983247cbea308c86c83f6f392.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات