این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۲۱، شماره ۴، صفحات ۳۹۱-۴۰۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Histological Evidence for Therapeutic Induction of Angiogenesis Using Mast Cells and Platelet-Rich Plasma within A Bioengineered Scaffold following Rat Hindlimb Ischemia
چکیده انگلیسی مقاله Objective
Peripheral arterial disease results from obstructed blood flow in arteries and increases the risk of amputation in acute cases. Therapeutic angiogenesis using bioengineered tissues composed of a chitosan scaffold that was enriched with mast cells (MCs) and/or platelet-rich plasma (PRP) was used to assess the formation of vascular networks and subsequently improved the functional recovery following hindlimb ischemia. This study aimed to find an optimal approach for restoring local vascularization.
Materials and Methods
In this experimental study, thirty rats were randomly divided into six experimental groups: a. Ischemic control group with right femoral artery transection, b. Ischemia with phosphate-buffered saline (PBS) control group, c. Ischemia with chitosan scaffold, d. Ischemia with chitosan and MCs, e. Ischemia with chitosan and PRP, and f. Ischemia with chitosan, PRP, and MCs. The left hind limbs served as non-ischemic controls. The analysis of capillary density, arterial diameter, histomorphometric analysis and immunohistochemistry at the transected locations and in gastrocnemius muscles was performed.
Results
The group treated with chitosan/MC significantly increased capillary density and the mean number of large blood vessels at the site of femoral artery transection compared with other experimental groups (P < 0.05). The treatment with chitosan/MC also significantly increased the muscle fiber diameter and the capillary-to-muscle fiber ratio in gastrocnemius muscles compared with all other ischemic groups (P < 0.05).
Conclusion
These findings suggested that chitosan and MCs together could offer a new approach for the therapeutic induction of angiogenesis in cases of peripheral arterial diseases.
کلیدواژه‌های انگلیسی مقاله Chitosan, Histology, Ischemia, Mast Cells, Platelet, Rich Plasma
نویسندگان مقاله Ali Karimi |
Department of Basic Science, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Rasoul Shahrooz |
Department of Basic Science, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Rahim Hobbenagh |
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Rahim Mohammadi |
Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Nowruz Delirezh |
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Saeede Amani |
Department of Basic Science, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Johan Garssen |
4Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, Netherlands;5Nutricia Research Centre for Specialized Nutrition, Utrecht, Netherlands

Ian M Adcock |
8Cell and Molecular Biology Group, Airways Disease Section, Faculty of Medicine, National Heart and Lung Institute, Imperial College London, London, United Kingdom ;9Priority Research Centre for Asthma and Respiratory Di

نشانی اینترنتی https://www.celljournal.org/article_250603_2b212369a227719e09259966116415aa.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات