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Cell Journal، جلد ۲۱، شماره ۳، صفحات ۳۱۴-۳۲۱
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عنوان انگلیسی Reduction of truncated Kit Expression in Men with Abnormal Semen Parameters, Globozoospermia and History of Low or Fertilization Failure
چکیده انگلیسی مقاله Objective
Phospholipase C zeta 1 (PLCζ) is one of the main sperm factor involved in oocyte activation and other factors may assist this factor to induce successful fertilization. Microinjection of recombinant tr-kit, a truncated form of c-kit receptor, into metaphase II-arrested mouse oocytes initiate egg activation. Considering the potential roles of tr- KIT during spermiogenesis and fertilization, we aimed to assess expression of tr-KIT in sperm of men with normal and abnormal parameters and also in infertile men with previous failed fertilization and globozoospermia.
Materials and Methods
This experimental study was conducted from September 2015 to July 2016 on 30 normozoospermic and 20 abnormozoospermic samples for experiment one, and also was carried out on 10 globozoospermic men, 10 men with a history low or failed fertilization and 13 fertile men for experiment two. Semen parameters and sperm DNA fragmentation were assessed according to WHO protocol, and TUNEL assay. Sperm tr- KIT was evaluated by flow cytometry, immunostaining and western blot.
Results
The results show that tr-KIT mainly was detected in post-acrosomal, equatorial and tail regions. Percentage of tr-KIT-positive spermatozoa in abnormozoospermic men was significantly lower than normozoospermic men. Also significant correlations were observed between sperm tr-KIT with sperm count (r=0.8, P < 0.001), motility (r=0.31, P=0.03) and abnormal morphology (r=-0.6, P < 0.001). Expression of tr-KIT protein was significantly lower in infertile men with low/ failed fertilization and globozoospermia compared to fertile men. The significant correlation was also observed between tr-KIT protein with fertilization rate (r=-0.46, P=0.04). In addition, significant correlations were observed between sperm DNA fragmentation with fertilization rate (r=-0.56, P=0.019) and tr-KIT protein (r=-0.38, P=0.04).
Conclusion
tr-KIT may play a direct or indirect role in fertilization. Therefore, to increase our insight regarding the role of tr-KIT in fertilization further research is warranted.
کلیدواژه‌های انگلیسی مقاله DNA fragmentation, Fertilization, Globozoospermia, Male infertility
نویسندگان مقاله Somayeh Haghighat |
Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran;Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran

Marziyeh Tavalaee |
Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran

Zahra Zakeri |
Department of Biology, Queens College and Graduate Center of the City University of New York, Flushing, NY, USA

Mahdi Noureddini |
Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran

Abdolhossein Shahverdi |
4Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Medicine, ACECR, Tehran, Iran

Mohammad Hossein Nasr Esfahani |
Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran;5Isfahan Fertility and Infertility Center, Isfahan, Iran

نشانی اینترنتی https://www.celljournal.org/article_250594_c560de23c6ae73ded68c3d185e3db5ee.pdf
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