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Cell Journal، جلد ۱۹، شماره ۴، صفحات ۵۵۹-۵۶۸
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عنوان انگلیسی Dual Effects of Cell Free Supernatants from Lactobacillus acidophilus and Lactobacillus rhamnosus GG in Regulation of MMP-9 by Up-Regulating TIMP-1 and Down-Regulating CD147 in PMA- Differentiated THP-1 Cells
چکیده انگلیسی مقاله Objective
Recent studies have reported dysregulated expression of matrix metalloproteinases (MMPs), especially MMP-2, MMP-9, tissue inhibitor of metalloproteinase-1, -2 (TIMP-1, TIMP-2), and extracellular matrix metalloproteinase inducer (EMMPRIN/CD147) in activated macrophages of patients with inflammatory diseases. Therefore, MMP-2, MMP-9, and their regulators may represent a new target for treatment of inflammatory diseases. Probiotics, which are comprised of lactic acid bacteria, have the potential to modulate inflammatory responses. In this experimental study, we investigated the anti-inflammatory effects of cell-free supernatants (CFS) from Lactobacillus acidophilus (L. acidophilus) and L. rhamnosus GG (LGG) in phorbol myristate acetate (PMA)-differentiated THP-1 cells.
Materials and Methods
In this experimental study, PMA-differentiated THP-1 cells were treated with CFS from L. acidophilus, LGG and uninoculated bacterial growth media (as a control). The expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 mRNAs were determined using real-time quantitative reverse transcription polymerase chain reaction (RT- PCR). The levels of cellular surface expression of CD147 were assessed by flow cytometry, and the gelatinolytic activity of MMP-2 and MMP-9 were determined by zymography.
Results
Our results showed that CFS from both L. acidophilus and LGG significantly inhibited the gene expression of MMP-9 (P=0.0011 and P=0.0005, respectively), increased the expression of TIMP-1 (P < 0.0001), decreased the cell surface expression of CD147 (P=0.0307 and P=0.0054, respectively), and inhibited the gelatinolytic activity of MMP-9 (P=0.0003 and P < 0.0001, respectively) in PMA-differentiated THP-1 cells. Although, MMP-2 expression and activity and TIMP-2 expression remained unchanged.
Conclusion
Our results indicate that CFS from L. acidophilus and LGG possess anti-inflammatory properties and can modulate the inflammatory response.
کلیدواژه‌های انگلیسی مقاله CD147, Inflammation, MMP, Probiotics, TIMP
نویسندگان مقاله Faezeh Maghsood |
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Abbas Mirshafiey |
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Mohadese M. Farahani |
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Mohammad Hossein Modarressi |
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Parvaneh Jafari |
Department of Microbiology, Science Faculty, Islamic Azad University, Arak Branch, Arak, Iran

Elahe Motevaseli |
5Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran

نشانی اینترنتی https://www.celljournal.org/article_250464_12b306d5cd8b443af22550bf150e60a1.pdf
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