این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۱۹، شماره ۳، صفحات ۳۳۲-۳۴۲
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Morphological and Molecular Aspects of In Vitro Culture of Preantral Follicles Derived from Vitrified Ovarian Tissues Using A Two-Step Culture
چکیده انگلیسی مقاله Objective
This study aimed to evaluate the expression of the genes related to folliculo-genesis after vitrification of mouse ovarian tissues using a two-step in vitro culture.
Materials and Methods
In this experimental study, vitrified and non-vitrified ovaries from 7- day old (neonate) female mice were cultured using alpha-Minimum Essential Medium (α-MEM) supplemented with 5% fetal bovine serum (FBS) for 7 days. Morphology, surface area of ovaries and percentage of normal follicles were evaluated and compared in both groups. After one-week culture, in non-vitrified group, preantral follicles of cultured ovaries were isolated and cultured in a three-dimensional alginate culture system for 12 days. Then, the collected metaphase (M) II oocytes were inseminated with capacitated spermatozoa derived from 7-8-week old (adult) male NMRI mice. Follicular diameter, oocyte maturation, fertilization, embryo development and the expression of genes related to follicular development (Pcna, Fshr and Cyp17a1,) using real time reverse transcription-polymerase chain reaction (RT-PCR) were assessed at the end of last culture period in both groups.
Results
The ovarian area in vitrified group (162468.20 703.78) was less than non-vitrified group (297211.40 6671.71), while the percentage of preantral follicles in vitrified group (18.40%) was significantly lower than those of non-vitrified group (24.50%) on day 7 of culture (P < 0.05). There were no significant differences between the two groups in terms of follicular diameter, expression of genes related to development of follicles, oocyte maturation, fertilization, as well as embryo development (P > 0.05).
Conclusion
The results of this study showed that vitrification of ovarian tissue following in vitro culture had negative impact on the survival and development of follicles within the tissue. However, no significant alterations were observed in development, gene expression and hormonal production of in vitro culture of isolated follicles derived from vitrified ovarian tissues as compared to the non-vitrified samples.
کلیدواژه‌های انگلیسی مقاله Vitrification, Folliculogenesis, Gene expression, Sodium alginate
نویسندگان مقاله Mahboobeh Amoushahi |
Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Mojdeh Salehnia |
Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Seyed Javad Mowla |
Department of Biotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

Nassim Ghorbanmehr |
Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran

نشانی اینترنتی https://www.celljournal.org/article_250440_7583414cb6811d574345542ed9e81e75.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات