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Cell Journal، جلد ۱۰، شماره ۳، صفحات ۱۹۳-۲۰۰
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چکیده فارسی مقاله
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عنوان انگلیسی Royana: Successful Experience In Cloning The Sheep
چکیده انگلیسی مقاله Objective: This study describes our experiences in reproductive cloning using two different procedures resulting in birth of the first successfully cloned sheep in Iran and the 
Middle-East, nick-named "Royana".
Materials and Methods: Abattoir-derived sheep oocytes were enucleated after in vitro 
maturation for 18-20hrs and then reconstructed by ear-derived sheep somatic cells using 
two different procedures of renucleation (subzonary, intracytoplasmic), embryo culture (coculture, sequential medium) and embryo transfer (intra fallopian, intra uterine). Pregnancy 
status and fetal development were followed regularly and elective cesarean was inducted 
on day 145 of pregnancy. Histopathological and genetical examinations were performed 
on either aborted and delivered clones for confirmation different aspects of cloning.
Results: The two procedures were both efficient in producing early and/or advanced 
cloned embryos, establishing early and/or advanced stages of pregnancy till delivery. Four 
pregnancies were detected; one were failed at early pregnancy, one aborted on day 90, 
one was still born and the fourth delivered to a healthy male lamb nick named "Royana".
Conclusion: Many different approaches have been developed for mammalian cloning 
which all are judged by their ultimate potency for establishment of successful pregnancies terminated to healthy/viable clones. As a preliminary study toward establishment of 
the technology, this study also successfully examined the competency of two procedures 
of somatic cell nuclear transfer (SCNT). However, the overall low efficiency of SCNT indicates that many different aspects of the technology remain to be dissolved.
کلیدواژه‌های انگلیسی مقاله Reproductive Cloning, Somatic Cell Nuclear Transfer (SCNT), Mammalian, Sheep
نویسندگان مقاله Saeed Kazemi Ashtiani |
Stem Cells Department, Cell Sciences Research Center, Royan Institute, ACECR, Tehran, Iran

Mohammad Hossein Nasr-Esfahani |
Clinical and Experimental Embryology Department, Cell Sciences Research Centre, Royan Institute, ACECR, Isfahan, Iran

Sayyed Mortaza Hosseini |
Clinical and Experimental Embryology Department, Cell Sciences Research Centre, Royan Institute, ACECR, Isfahan, Iran

Fariba Moulavi |
Clinical and Experimental Embryology Department, Cell Sciences Research Centre, Royan Institute, ACECR, Isfahan, Iran

Mahdi Hajian |
Clinical and Experimental Embryology Department, Cell Sciences Research Centre, Royan Institute, ACECR, Isfahan, Iran

Mohsen Frouzanfar |
Faculty of Basic Science, Islamic Azad University, Marvdasht Branch, Iran

Parvaneh Abedi |
Clinical and Experimental Embryology Department, Cell Sciences Research Centre, Royan Institute, ACECR, Isfahan, Iran

Maryam Meamar |
Clinical and Experimental Embryology Department, Cell Sciences Research Centre, Royan Institute, ACECR, Isfahan, Iran

Mojtaba Rezazadeh Valojerdi |
Department of Clinical and Experimental Embryology, Reproductive Medicine Research Centre, Royan Institute, ACECR, Tehran, Iran

Hamid Gourabi |
Stem Cells Department, Cell Sciences Research Center, Royan Institute, ACECR, Tehran, Iran

Abdolhossein Shahverdi |
Stem Cells Department, Cell Sciences Research Center, Royan Institute, ACECR, Tehran, Iran

Hossein Baharvand |
Stem Cells Department, Cell Sciences Research Center, Royan Institute, ACECR, Tehran, Iran

Ahmad Vosough Dizaj |
Stem Cells Department, Cell Sciences Research Center, Royan Institute, ACECR, Tehran, Iran

Hossein Imani |
Department of Clinical and Experimental Embryology, Reproductive Medicine Research Centre, Royan Institute, ACECR, Tehran, Iran

Poopak Eftekhari-Yazdi |
Department of Clinical and Experimental Embryology, Reproductive Medicine Research Centre, Royan Institute, ACECR, Tehran, Iran

Mahdi Vojgani |
Clinical Sciences Department, Faculty of Veterinary, Tehran University, Tehran, Iran

Mahdi Safahani |
Obstetric and Gynecology Unit, Fooka Dairy Complex, Social Security Association, Isfahan, Iran

Ramin Radpour |
Genetic Department, Reproductive Medicine Research Centre, Royan Institute, ACECR, Tehran, Iran

Iman Salahshouri |
Genetic Department, Reproductive Medicine Research Centre, Royan Institute, ACECR, Tehran, Iran

نشانی اینترنتی https://www.celljournal.org/article_248199_48a6866fe71907098c23356fa3f2cd51.pdf
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