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International Journal of Fertility and Sterility، جلد ۱۴، شماره ۲، صفحات ۸۴-۹۰
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عنوان انگلیسی The Effects of Calligonum Extract and Low-Intensity Ultrasound on Motility, Viability, and DNA Fragmentation of Human Frozen-Thawed Semen Samples
چکیده انگلیسی مقاله Background
The study aimed to evaluate the impact of Calligonum extract and US radiation on sperm parameters of cryopreserved human semen samples.
Materials and Methods
In this experimental study, twenty-five semen specimens were obtained from healthy semen donors and incubated in human tubal fluid (HTF) medium supplemented with 10% human serum albumin (HSA) for 45 minutes. Samples were treated with Calligonum extract (10 μg/ml) alone (CGM group) and US radiation (LIPUS- exposed group) alone or a combination of both treatments (CGM+LIPUS). The US group received US stimulation (in both continuous and pulsed wave modes) at a frequency of 1 MHZ and intensity of 200 mW/cm2 for 200 seconds. Sperm morphology was assessed by Diff-Quik staining. The DNA fragmentation was evaluated the Halo sperm kit. Sperm parameters was analyzed by a computer-assisted semen analysis system. Reactive oxygen species (ROS) was assessed by flow cytometry.
Results
The results showed that the treatment with Calligonum extract significantly (P < 0.05) increased the progressive motility of spermatozoa in the CGM group as compared with the control group. The application of low-intensity US significantly (P < 0.05) decreased the motility and viability of spermatozoa in the US group when compared with the control group. Our findings also indicated that the use of both low-intensity US in continuous mode and Calligonum extract slightly increased progressive motility; however, such an increase was not statistically significant. The rate of DNA fragmentation was considerably higher (P < 0.05) in control and LIPUS-exposed groups than the other groups.
Conclusion
Treatment of spermatozoa with Calligonum extract slightly improved the sperm parameters due to its antioxidant activity, on the other hand, according to our results, US radiation did not improve sperm parameters which may be due to interference with the motility of sperm, as well as its physical effects on spermatozoa.
کلیدواژه‌های انگلیسی مقاله antioxidants, Cryopreservation, low, intensity ultrasound, Spermatozoa
نویسندگان مقاله Hamid Ekrami |
Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran;Basic Medical Science Research Center, Histogenotech Company, Tehran, Iran

Mansoureh Movahedin |
Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Fereshteh Koosha |
Department of Radiology Technology, Faculty of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Zohreh Mazaheri |
Basic Medical Science Research Center, Histogenotech Company, Tehran, Iran

Manijhe Mokhtari-Dizaji |
4Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

نشانی اینترنتی https://www.ijfs.ir/article_45586_d89b29701c61a2716710171c6778008f.pdf
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