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International Journal of Fertility and Sterility، جلد ۱۴، شماره ۲، صفحات ۱۱۰-۱۱۵
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چکیده فارسی مقاله
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عنوان انگلیسی Interfering Effects of In Vitro Fertilization and Vitrification on Expression of Gtl2 and Dlk1 in Mouse Blastocysts
چکیده انگلیسی مقاله Background
Embryo vitrification is a key instrument in assisted reproductive technologies (ARTs). However, there is increasing concern that vitrification adversely affects embryo development. This study intends to assess the effect of vitrification on developmental competence, in addition to expressions of long non-coding RNA (lncRNA) gene trap locus 2 (Gtl2) and its reciprocal imprinted gene delta-like homolog 1 (Dlk1), in mouse blastocysts.
Materials and Methods
In this experimental study, we have designed three experimental groups: control (fresh blas- tocysts collected from superovulated mice), in vitro fertilization (IVF; blastocysts derived from IVF) and vitrification (IVF derived blastocysts subjected to vitrification/warming at the 2-cell stage). Quantitative reverse transcription pol- ymerase chain reaction (qRT-PCR) was performed to assess the expression levels of Gtl2 and Dlk1 in the blastocysts.
Results
The results showed that vitrification group had significantly lower blastocyst and hatching rates compared to the IVF group (P < 0.037) and (P < 0.041), respectively. Gtl2 was down-regulated and Dlk1 was up-regulated following the IVF and vitrification (P < 0.05).
Conclusion
These results suggested that IVF and vitrification disturbed genomic imprinting and lncRNA gene expres- sions, which might affect the health of IVF children.
کلیدواژه‌های انگلیسی مقاله IVF, Mouse, Preimplantation Embryo, Vitrification
نویسندگان مقاله Elham Movahed |
Department of Anatomical Sciences, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

Ronak Shabani |
Department of Anatomical Sciences, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran;Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran

Sara Hosseini |
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Solmaz Shahidi |
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran;4Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences

نشانی اینترنتی https://www.ijfs.ir/article_45589_bacc6cdecda489cdb5f69139085c9a67.pdf
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