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International Journal of Fertility and Sterility، جلد ۱۴، شماره ۲، صفحات ۱۲۲-۱۲۹
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عنوان انگلیسی Phosphodiesterase 8B Polymorphism rs4704397 Is Associated with Infertility in Subclinical Hypothyroid Females: A Case-Control Study
چکیده انگلیسی مقاله Background
Subclinical hypothyroidism (SCH) remains largely unnoticed as a major cause of infertility due to asymptomatic. Polymorphisms of phosphodiesterase 8B gene (PDE8B) have been linked with various diseases, including female infertility. Hence, we aimed to study prevalence of SCH, in infertile females, explore associa- tion of PDE8B rs4704397 A/G and rs6885099 G/A polymorphisms with infertility in females suffering from SCH and genotype-phenotype correlation of the polymorphisms with thyroid stimulating hormone (TSH) levels in Gujarat population.
Materials and Methods
In this retrospective study, TSH level was estimated from plasma of 230 infertile and 100 control females by enzyme-linked fluorescence immunoassay (ELFA) to find out the prevalence of SCH. Further, based on TSH levels, thyroid function test (TFT) was performed in controls and infertile females with subclinical hypothyroidism (IF-SCH). PDE8B rs4704397 and rs6885099 polymorphisms were genotyped by PCR-RFLP and ARMS-PCR, respectively in 74 controls and 60 IF-SCH females.
Results
We observed i. significantly high prevalence of SCH (32%) in the infertile females, ii. significant- ly lower frequency of ‘G’ allele (P=0.006), while the frequency of ‘A’ allele (P < 0.0001) was higher in IF- SCH females, compared to the controls, for rs4704397 A/G SNP, iii. no significant difference in the genotype (P=0.214; OR=2.51; CI=0.74–8.42) and the allele frequency (P=0.129; OR=1.51; CI=0.92-2.47) of rs6885099 G/A SNP, iv) low linkage disequilibrium for the polymorphisms, v. significantly higher frequency of ‘AA’ hap- lotype (P=0.0001; OR=3.84; CI=1.86-8.01),while the ‘GG’ haplotype (P=0.0023; OR=0.33; CI=0.16-0.69) was significantly lower in IF-SCH females and vi. no significant difference in the TSH level of IF-SCH females with respect to the genotypes.
Conclusion
The present study reports an association of PDE8B rs4704397 polymorphism with infertility in SCH females. The study categorically shows a higher prevalence of SCH in infertile females of Gujarat and advocates the importance of screening for SCH in infertility management.
کلیدواژه‌های انگلیسی مقاله Genetic polymorphisms, infertility, Thyroid
نویسندگان مقاله Tabassum Mansuri |
Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Gujarat, India

Shahnawaz D. Jadeja |
Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Gujarat, India

Mala Singh |
Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Gujarat, India

Rasheedunnisa Begum |
Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Gujarat, India

Pushpa Robin |
Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Gujarat, India

نشانی اینترنتی https://www.ijfs.ir/article_45591_dbd3fedecd5b4e853a27602d4e98796b.pdf
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