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International Journal of Fertility and Sterility، جلد ۱۴، شماره ۲، صفحات ۱۴۳-۱۴۹
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عنوان انگلیسی Effect of Vitamin D3 on Mitochondrial Biogenesis in Granulosa Cells Derived from Polycystic Ovary Syndrome
چکیده انگلیسی مقاله Background
Polycystic ovary syndrome (PCOS) is an endocrine disorder diagnosed by anovulation hyperandro- genism. Hyperandrogenism increases apoptosis, which will eventually disturb follicular growth in PCOS patients. Since mitochondria regulate apoptosis, they might be affected by high incidence of follicular atresia. This may cause infertility. Since vitamin D3 has been shown to improve the PCOS symptoms, the aim of study was to investigate the effects vitamin D3 on mtDNA copy number, mitochondrial biogenesis, and membrane integrity of granulosa cells in a PCOS-induced mouse model.
Materials and Methods
In this experimental study, the PCOS mouse model was induced by dehydroepiandrosterone (DHEA). Granulosa cells after identification by follicle-stimulating hormone receptor (FSHR) were cultured in three groups: 1. granulosa cells treated with vitamin D3 (100 nM for 24 hours), 2. granulosa cells without any treatments, 3. Non-PCOS granulosa cells (control group). Mitochondrial biogenesis gene (TFAM) expression was compared between different groups using real-time PCR. mtDNA copy number was also investigated by qPCR. The mitochon- drial structure was evaluated by transmission electron microscopy (TEM). Hormonal levels were measured by an enzymelinked immunosorbent assay (ELISA) kit.
Results
The numbers of pre-antral and antral follicles increased in PCOS group in comparison with the non-PCOS group. Mitochondrial biogenesis genes were downregulated in granulosa cells of PCOS mice when compared to the non-PCOS granulosa cells. However, treatment with vitamin D3 increased mtDNA expression levels of these genes compared to PCOS granulosa cells with no treatments. Most of the mitochondria in the PCOS group were spherical with almost no cristae. Our results showed that in the PCOS group treated with vitamin D3, the mtDNA copy number increased significantly in comparison to PCOS granulosa cells with no treatments.
Conclusion
According to this study, we can conclude, vitamin D3 improves mitochondrial biogenesis and membrane integrity, mtDNA copy number in granulosa cells of PCOS mice which might improve follicular development and subsequently oocyte quality.
کلیدواژه‌های انگلیسی مقاله Granulosa cell, Mitochondrial Biogenesis, Mitochondrial DNA, Polycystic Ovary Syndrome, Vitamin D3
نویسندگان مقاله Zahra Safaei |
Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran

Mohammad Hossein Nasr Esfahani |
5Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran

Azadeh Akbari Sene |
6Shahid Akbarabadi Clinical Research Development Unit (SHACRDU), Iran University of Medical Sciences (IUMS), Tehran, Iran

Vahid Najafzadeh |
7Department of Veterinary and Animal Sciences, Anatomy and Biochemistry Section, University of Copenhagen, Copenhagen, Denmark

Mansoureh Soleimani |
Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

Reza Shirazi |
Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran;8Department of Health and Medical S

Shabnam Bakhshalizadeh |
Reproductive Development, Murdoch Children's Research Institute, Melbourne, Victoria, Australia;4Department of Paediatrics, University of Melbourne, Melbourne, Victoria, Australia

نشانی اینترنتی https://www.ijfs.ir/article_45594_de8c9880e187ba88d5c58f3b25f44b16.pdf
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