این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Tanaffos، جلد ۲۰، شماره ۴، صفحات ۳۵۳-۳۶۲
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی A Lysine-Functionalized Graphene Oxide-Based Nanoplatform for Delivery of Fluorouracil to A549 Human Lung Cancer Cells: A Comparative Study
چکیده انگلیسی مقاله Background: Today, increasing attention is being paid to the application of biocompatible polymers as drug carriers with low cytotoxicity in drug delivery systems to enhance the therapeutic effects of anticancer agents. Materials and Methods: In this study, a biocompatible synthetic polymer (grafted on graphene oxide), composed of N-isopropylacrylamide and 1-vinyl-2-pyrrolidone with L-lysine segments (Lys/PNIPAM-PVP/GO), was developed as a nano-vehicle for the drug. This platform was used for the delivery of fluorouracil (FU) to A549 human lung cancer cells. The superior characteristics of the platform included low-cost precursors, easy synthesis, and the presence of many functional groups for loading drugs. To determine and compare the cytotoxic effects of free FU and its formulated form on the A549 cells, MTT assay was performed; the results showed no significant toxicity difference between the two treated groups (free and formulated FU). For further evaluations, cellular uptake assays were performed via fluorescence microscopy and flow cytometry. Results: Both analyses revealed the low internalization of nano-vehicle into the A549 cells, with 4.31% and 8.75% cellular uptakes in the first two and four hours of treatment. Therefore, the low penetration rate reduced the toxicity of drug-loaded nano-vehicle. Conclusion: Finally, DAPI staining and Annexin V-FITC staining were performed as complementary techniques to determine cell apoptosis
کلیدواژه‌های انگلیسی مقاله Graphene oxide,drug carrier,Fluorouracil,A549 cell line,Lung cancer
نویسندگان مقاله Maryam Ashjaran |
Department of Chemistry, Tabriz Branch, Islamic Azad University, Tabriz, Iran

Mirzaagha Babazadeh |
Department of Chemistry, Tabriz Branch, Islamic Azad University, Tabriz, Iran

Abolfazl Akbarzadeh |
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Soodabeh Davaran |
Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

Roya Salehi |
Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

نشانی اینترنتی https://www.tanaffosjournal.ir/article_254209_7ebf79a8b0530733b733ad09067f1148.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات