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Tanaffos، جلد ۱۸، شماره ۲، صفحات ۸۹-۹۵
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عنوان انگلیسی Developing Rat Bone Marrow Derived Mast Cells by the Splenic Cells Culture Supernatant of Rat and Mouse
چکیده انگلیسی مقاله Background: Mast cells play a critical role in the pathogenesis of various immunological and non-immunological diseases. It is now accepted that culturing primary mast cells considered as a tool for investigation role of mast cells in diseases. Development of various animal primary mast cells and their function could be used for the translational studies in the pathogenesis of human diseases. The aim of the study was to develop simple and cost-efficient method for differentiation and culture of rat mast cells from bone marrow by using rat and mouse spleen supernatant. Materials and Methods: Bone marrow cells from 10 to15-weeks-old male rats was obtained and cultured for three weeks on cell culture medium. After that, purity of cells was approved by FCԑRI and CD117 antibodies, toluidine blue and Immunohistochemistry (IHC). Results: After 3 weeks continuous culturing, high purity of cells was found. CD117, CD34 expression and tryptase were 80.1, 76.89 and 87.9%, respectively by rat splenic supernatant, whereas 85.4, 83.07 and 82.1%, respectively with mouse splenic supernatants. Besides, rat spleen supernatant developed 91.4% and mouse splenocyte supernatant developed 89.7% mast cells based on surface markers. Conclusion: The data presented in this study indicated equal maturation and differentiation of bone marrow derived rat mast cells by using both spleen supernatants.
کلیدواژه‌های انگلیسی مقاله Bone marrow,Mast cell differentiation,Rat splenic cell culture
نویسندگان مقاله Saeede Amani |
Department of Comparative Histology and Anatomy Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran,

Rasoul Shahrooz |
Department of Comparative Histology and Anatomy Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran,

Ali Karimi |
Department of Comparative Histology and Anatomy Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran,

Zahra Bakhtiari |
Department of Comparative Histology and Anatomy Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran,

Esmaeil Mortaz |
Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran,

نشانی اینترنتی https://www.tanaffosjournal.ir/article_239984_1a8ee11c332cd6c1411e94afbf8f0c6f.pdf
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