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Iranian Journal of Pathology، جلد ۱۸، شماره ۳، صفحات ۳۵۶-۳۶۲
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عنوان انگلیسی Simultaneous Genetically Detection of Streptococcus pyogenes, Streptococcus pneumoniae and Haemophilus influenzae in Patients with Treatment-Resistant Respiratory Infection
چکیده انگلیسی مقاله Background & Objective: Streptococcus pneumoniae, Haemophilus influenzae and Streptococcus pyogenes are among the most important causes of infection in human. Inventing rapid methods to identify these species can help in providing appropriate and effective treatment options. Therefore, the current study aimed to develop a multiplex touch-down PCR method to identify rapidly the aforementioned species patients' sputum samples, simultaneously.
Methods: A total of 50 sputum samples of patients with respiratory infections resistant to treatment were collected. After DNA extraction and primer design, the complete capsule (CAP) region II, capsular polysaccharide biosynthesis (cpsA) and the structural regulator of transcription (spy) genes were amplified for detecting H. influenzae, S. pneumoniae and S. pyogenes by multiplex touch-down PCR.
Results: Among 50 samples prepared from patients with different diseases, 27 samples were positive for amplified genes. The frequency of presence of pathogens in the collected samples included 14% H. influenzae, 20% S. pneumoniae and 20% S. pyogenes. Also, in some patients, the simultaneous presence of two or three pathogens were observed.
Conclusion: In general, it can be concluded that the PCR touchdown method developed in the present study is an effective and fast method for the simultaneous identification of H. influenzae, S. pneumoniae and S. pyogenes pathogens in clinical samples of patients.
کلیدواژه‌های انگلیسی مقاله gene,Multiplex PCR,Pathogen,Sputum
نویسندگان مقاله Farzad Mohammadi Ebli |
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Zoheir Heshmatipour |
Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran

Khadijeh Daneshjou |
Department of Pediatrics, Imam Khomeini Hospital, School of Medicine, Tehran University of Medical Science, Tehran, Iran

Seyed Davood Siadat |
Department of Mycobacteriology and Pulmonary Research, Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran

نشانی اینترنتی https://ijp.iranpath.org/article_707319_b8cc6d963f5ea6fb991e9d9c0cf9c003.pdf
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