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Iranian Journal of Pathology، جلد ۱۷، شماره ۴، صفحات ۴۴۸-۴۶۰
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عنوان انگلیسی Comparison of Immune Response in Mice Immunized with Recombinant PreS2/S-C18-27 Protein Derived from Hepatitis B Virus with Commercial Vaccine
چکیده انگلیسی مقاله Background & Objective: The vaccine available to prevent Hepatitis B virus disease is ineffective in 5% of people due to the use of HBsAg as a weak immunogen. In the present study, PreS2/S fused to C18-27 peptide fragment as an effective antigen and is proposed as a promising vaccine candidate compared with the conventional vaccine prescribed in the vaccination program.
Methods: After the synthesis of PreS2/S genes and C18-27 peptide fragment in pET28a, the recombinant protein was confirmed by Western blotting. The efficacy of the PreS2/S-C18-27 protein was compared with the conventional vaccine injected into five groups of rats. Finally, the cytokine level of IF-r, IL-2, IL-4, IL-10, TNF-a, IgG1, and IgG2a were measured using the ELISA method.
Results: This study showed no significant difference between the recombinant vaccine group and PBS control group in the IF-r test, but there was a significant difference between groups testing IL-2 and IL-10. In addition, the group receiving the recombinant vaccine with CPG adjuvant at a dilution of 1/10 in the IgG total test on days 14 and 45 after the first injection showed a significant difference in comparison with other groups.
Conclusion: This study showed no statistically significant difference between the recombinant protein vaccine group and the conventional vaccine group. The Th1- mediated immune responses obtained from recombinant proteins with and without CPG performed better than conventional vaccines, possibly due to the functional deficiency of the available vaccines.
کلیدواژه‌های انگلیسی مقاله CpG 7909,Hepatitis B Core Antigen,Hepatitis B Vaccines,Hepatitis B virus,preS2HBsAg
نویسندگان مقاله Elaheh Gholami Parizad |
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran

Abbas Ali Imani Fooladi |
Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

Hamid Sedighian |
Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

Elham Behzadi |
Academy of Medical Sciences of the I.R. of Iran, Tehran, Iran

Azar Valizadeh |
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran

Afra Khosravi |
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran

نشانی اینترنتی https://ijp.iranpath.org/article_254533_15cd5c6d53a51c9ad6c852bd0ea36c88.pdf
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