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Iranian Journal of Pathology، جلد ۱۷، شماره ۳، صفحات ۳۰۳-۳۱۳
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عنوان انگلیسی Evaluation of Triple Fragment Vaccine HSPX (Rv2031c) + PPE44 (Rv2770c) + Mouse IgG1 (Fcγ2a) with Auxiliary Adjuncts IL-22 in Comparison with BCG Vaccine
چکیده انگلیسی مقاله Background & Objective: Despite the vaccination with the BCG vaccine, tuberculosis (TB) remains one of the major health problems in the world. The aim of this study was to evaluate our newly designed vaccine using IL-22 as an adjuvant in comparison with the common BCG vaccine.
Methods: The gene constructs were cloned into the expression vector of pET28a and then into the recombinant vector of PET28a – HSPX, and PPE44 was transformed into Escherichia coli BL21 (DE3). Finally, the immunogenicity of recombinant proteins with and without BCG and IL-22 in BALB/c mice was investigated.
Results: The key cytokines INF-γ and TNF-α were elevated more greatly in BCG immunized group than in PHF immunized group.
Immunization with PHF showed a significant increase in IL-4 levels versus the BCG group. Adding IL-22 to the vaccine formulations indicated a tiny increase in IL-4 levels compared to their related vaccine groups.
Specific total IgG1 in the experimental groups showed an increase in comparison with control groups, but in the vaccinated groups, no significant differences were observed, and the presence of IL-22 in the vaccine formulations indicated a slight decrease compared with the related mere vaccine groups. Results of specific total IgG2a in the experimental groups revealed that only in the PHF group formulated with IL-22 a significant increase occurs compared with all other experimental groups.
Conclusion: It seems that BCG, as the only licensed vaccine for TB infection, could be more potent than a recombinant vaccine in the induction of cellular and humoral immune responses.
کلیدواژه‌های انگلیسی مقاله Subunit,PPE44,HSPX,IL-22
نویسندگان مقاله Azar Valizadeh |
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.

Fra Khosravi |
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran

Hamid Sedighian |
Applied Microbiology Research Center, Systems biology and poisonings institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

Elham Behzadi |
Academy of Medical Sciences of the IR of Iran, Tehran, Iran

Elaheh Parizad |
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.

Abbas Ali Imani Fooladi |
Applied Microbiology Research Center, Systems biology and poisonings institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

نشانی اینترنتی https://ijp.iranpath.org/article_254067_2019d02d0291ef14405139a32c95d328.pdf
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