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Iranian Journal of Pathology، جلد ۱۷، شماره ۱، صفحات ۲۹-۳۶
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عنوان انگلیسی LncRNA SNHG6 Silencing Could Arrest Progression of High Grade Colorectal Cancers
چکیده انگلیسی مقاله Background & Objective: Colorectal cancer (CRC), like other cancers, needs faster and more accurate identifications. A well-timed prognosis of CRC could be an important turning point in the survival of patients. Supplementary signs, such as long non-coding RNAs (lncRNAs), could be helpful for this purpose. A new possible biomarker for CRC identification is introduced by this study.
Methods:  RNA extraction was performed by the RNX-Plus solution for 64 tumor and non-tumor tissues. Complementary DNAs (cDNAs) were synthesized, and quantitative real-time PCR was performed for relative expression level measurement and the data was analyzed statistically using the Prism 6 software. For Small nucleolar host gene 6 knockdown, siRNA was designed based on Reynolds rules. The cells were cultured in their appropriate media, and the siRNA-lipofectamine complex was formed. The transfection complex was presented for sw48, sw480, and sw1116 as CRC cells with different grades. After transfection, the SNHG6/β actin ratio was determined. Then, the distribution of siRNA-treated cells was determined by the Partec flow cytometer instrument and analyzed by the FloMax software.
Results: SNHG6 was more expressed in CRC tumors than non-tumor tissues. In tumor tissues, SNHG6 upregulation and tumors’ grade progression were concurrent. SNHG6 was upregulated in cases with lymphovascular invasion than in cases with perineural invasion. The knockdown of SNHG6 conduced to G1 arrest in CRC cells, more noticeably in high-grade ones.
Conclusion: SNHG6 could be applied as a consideration to differentiate tumor and non-tumor tissues and grade definition in colorectal malignancies, and it could participate in colorectal tumor formation as a cell cycle progressive factor.
کلیدواژه‌های انگلیسی مقاله Cell cycle, Colorectal cancer, Long non-coding RNAs, 20.1001.1.17355303.2022.17.1.5.4, Main Subjects GI, Liver &,amp Pancreas Pathology, IntroductionCancer is a complicated disease accompanied by genetic and/or metabolic causes (1,, 2,). Colorectal cancer (CRC) is one of the frequent cancers (3,). Like many cancers, CRC has a specific expression pattern of long non-coding RNAs (lncRNAs) in its transcriptome. Non-coding RNAs (ncRNAs) as new players of cancer progression have become increasingly interested in the last decade lncRNAs are important and famous members of this category (4,). It has been proved that many tumor tissues alter the expression level of lncRNAs compared to their respective non-tumor marginal tissues. Thus, the expression of these transcripts could be an important sign for prognosis and diagnosis of cancers (5,-7,).Some lncRNAs are upregulated in CRC. These RNAs act via different pathways for the progression of cancer in colorectal tissue (8,). One of the lncRNA families that affect the colorectal tissue is the small nucleolar host gene (SNHG) family. This family has many members, such as SNHG1, SNHG3, SNHG5, SNHG7, SNHG12, SNHG15, and SNHG20. The important characteristic of this family is the presence of short sequences of small nucleolar RNAs (snoRNAs) in their sequence. Because of this characteristic, they are called snoRNA host genes (9,). CharacteristicNumberGrade I 10 (31.2 %) Grade II 9 (28.1 %) Grade III 5 (15.6 %) Grade IV 3 (9 %) Tumors with unknown grade 5 (15.6 %) Stage II 12 (37.5 %) Stage III 12 (37.5 %) Stage IV 2 (6 %) Tumors with unknown stage 6 (18.7 %) Tumor size
نویسندگان مقاله Amin Jafari Oliayi |
Pathology and Stem Cell Research Center, Department of Pathology, Afzalipour Medical School, Kerman University of Medical Sciences, Kerman, Iran

Shahriar Dabiri |
Pathology and Stem Cell Research Center, Department of Pathology, Afzalipour Medical School, Kerman University of Medical Sciences, Kerman, Iran

Malek Hossein Asadi |
Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology Kerman, Iran

نشانی اینترنتی https://ijp.iranpath.org/article_248591_5b881c5439efe2d6c0e6d04d0967270b.pdf
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