این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Research Journal of Pharmacognosy، جلد ۸، شماره ۳، صفحات ۳۳-۴۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Holothurin B Isolated from Holothuria atra Inhibits Angiogenesis More Potent than Curcumin in Vitro
چکیده انگلیسی مقاله Background and objectives: Triterpene glycosides as the most bioactive components of sea cucumbers, have been considered for their various pharmacological properties especially anticancer and anti-metastasis activities. Due to the limited information on the biological properties of holothurin B as a marine triterpene glycoside, the present study aimed to examine its effect on angiogenesis and compare it with curcumin usinghuman umbilical vein endothelial cells (HUVECs). Methods: Holothurin B was isolated from Holothuria atra and identified by NMR and Mass spectroscopic data. Cell survival was estimated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) technique and migration of cells was assessed by Transwell test. Angiogenesis was evaluatedin vitro by tube formation assay. Results: Holothurin B reduced HUVECssurvival with IC50 value of 8.16 µg/mL. At the concentrations of 5 and 7.5 µg/mL, it significantly decreased the number of migrated cells, the average length and size of tubules, and mean number of junctions; it was more potent than curcumin. Conclusion: Holothurin B could be considered as a potent antiangiogenic constituent through suppressing endothelial cell proliferation, migration and tubulogenesis in vitro, suggesting its potential for further animal and clinical investigations.
 
کلیدواژه‌های انگلیسی مقاله angiogenesis inhibitors,cell migration inhibition,Cell proliferation,holothurin B,human umbilical vein endothelial cells
نویسندگان مقاله Afsaneh Yegdaneh |
Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

Leila Safaeian |
Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

Mina Mirian |
Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

Nasim Dana |
Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.

Mohadeseh Taheri |
Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

نشانی اینترنتی http://www.rjpharmacognosy.ir/article_130034_295f1fe86421bcce0f9aab21a2117316.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات