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Research Journal of Pharmacognosy، جلد ۷، شماره ۴، صفحات ۷۵-۸۲
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عنوان انگلیسی Evaluation of the Cytotoxicity Activity of Gypsophila ruscifolia by Bioassay-Guided Fractionation
چکیده انگلیسی مقاله Background and objectives: The second cause of death in the world and the third cause in Iran is cancer which requires special attention for treatment. The previous reports of Gypsophila genus show significant toxic effects on different cancer cell lines. In this study, bioassay-guided fractionation was applied to determine the cytotoxic activity of root and aerial parts extracts and fractions of Gypsophila ruscifolia Methods: n-Hexane, chloroform, and methanol: H2O (8:2) extracts of root and aerial parts were prepared. Inhibition of cell growth determined by MTT assay in MCF-7, HT-29, A-549, and AGO-1522 cell lines. The most effective extract was fractionated by column chromatography. The cytotoxic effect of fractions was evaluated by MTT assay and apoptotic property of the cytotoxic fraction was determined by annexin V/PI assay in MCF-7 cell line. Results: The chloroform root extract of G. ruscifolia showed cytotoxicity in MCF-7 cells with IC50 value of 111.6 ±11.78 μg/mL. MTT assay of five of fractions demonstrated that F3 and F4 with IC50 values of 73.09 ±14.22 and 67.98 ±15.31 μg/mL on MCF-7 cell line, respectively showed cytotoxic effects. Also, F4 demonstrated apoptotic potential in MCF-7 cell line. Conclusion: Considering the results of cytotoxicity and apoptosis studies, isolation and identification of responsible compounds in the chloroform root extract of Gypsophila ruscifolia can be useful in cancer research studies.
کلیدواژه‌های انگلیسی مقاله Apoptosis,cell survival,Cell line,Gypsophila ruscifolia,medicine plant
نویسندگان مقاله Marzie Kamali |
Department of Pharmacognosy, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Mahmoud Mosaddegh |
Department of Pharmacognosy, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Mohammad-Reza Delnavazi |
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

Roksana Shahrestani |
Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Maryam Malek Mohammadi |
Department of Plant Sciences, School of Biology, College of Sciences, University of Tehran, Tehran, Iran.

Maryam Hamzeloo-Moghadam |
Department of Traditional Pharmacy and Traditional Medicine and Materia Medica Research Center, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

نشانی اینترنتی http://www.rjpharmacognosy.ir/article_114253_80d9e7168566eb08b8cc34fa88f58d6a.pdf
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