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Analytical and Bioanalytical Electrochemistry، جلد ۱۴، شماره ۱۱، صفحات ۱۰۲۷-۱۰۴۳
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عنوان انگلیسی Calibration Curve Approaches for Nonlinear Data Points Obtained in Colo 320 Exosomes Determination
چکیده انگلیسی مقاله The limit of detection (LOD) is defined as the lowest quantity or concentration of a component that can be reliably distinguished from the limit of blank (LOB). LOD value is one of the most important parameters considered for many determination methods and is usually calculated on the linear correlation between signal and concentration. However, the linear correlation may not always be obtained in experimental studies. We claim that data with low linear correlation have meaning, and we present such a study because analytical studies based on these data are not easily understood in the literature. In this manuscript, we suggest that a calibration curve can be obtained from nonlinear data points and the LOD value can be calculated. We tested this approach for the determination of exosomes and supported it with mathematical calculations. We produced a label-free sensor using anti-CD63 on the gold electrode for selective and reliable impedimetric detection of the exosomes obtained from Colo 320 cell lines in data points that are high concentrations and out of linearity. We characterized in detail what each calculation means. This sensor with a LOD value of 3.90×1011 exosome particles µL-1 and with a cubic polynomial model for the calibration curve was considered sensitive and reliable, especially for high vesicle content of samples such as cell culture medium.
کلیدواژه‌های انگلیسی مقاله Nonlinear calibration curve,Exosome determination,electrochemical impedance spectroscopy,Mathematical models, Biosensors
نویسندگان مقاله Sevda Akay Sazaklioglu |
Department of Medical Services and Techniques, Ankara Medipol University, 06050, Ankara, Turkey

Hilal Torul |
Faculty of Pharmacy, Department of Analytical Chemistry, Gazi University, 06330, Ankara, Turkey

Hilal Kabadayi |
Department of Histology and Embryology, Manisa Celal Bayar University, 45030, Manisa, Turkey

Hafize Seda Vatansever |
Department of Histology and Embryology, Manisa Celal Bayar University, 45030, Manisa, Turkey

Ugur Tamer |
Faculty of Pharmacy, Department of Analytical Chemistry, Gazi University, 06330, Ankara, Turkey

Huseyin Celikkan |
Faculty of Science, Department of Chemistry, Gazi University, 06560, Ankara, Turkey

نشانی اینترنتی https://www.abechem.com/article_698015_f4fe9fac504f669c7615ca3830bd1eb0.pdf
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